Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 15, Problem 3NST
The cancer drug melphalan is an alkylating agent of the mustard gas family. It acts in two ways: by causing alkylation of guanine bases and by cross linking DNA strands together. Describe two ways in which melphalan might kill cancer cells. What are two ways in which cancer cells could repair the DNA-damaging effects of melphalan?
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Analogs of nucleotides are often used in studies of DNA. An analog is a modification of one of the normal bases that can be incorporated into DNA via replication and can later be detected for analysis. The thymidine analog 5-ethynyl-2′-deoxyuridine (EdU) is widely used in the laboratory for these types of experiments. However, EdU is toxic to cells, and inclusion of EdU in growth medium eventually causes cell death, unlike other thymidine analogs.
Recently, a research team examined if cells recognized EdU as damage to the DNA and if the cells used the nucleotide excision repair (NER) systems to fix the DNA (see figure A). Human cells cultured in the lab were treated for 24 hours with either EdU or another thymidine analog, BrdU (blue circles in the figure). The cells were then exposed to UV light where damage would occur (red triangles in the figure). After 1 h of repair in the presence of the analog, the cells were lysed and excision products were separated from cells. An…
Some antibiotic drugs fight infection by interfering with DNA replication, transcription, or translation in bacteria. Indicate whether each of the following antibiotic drug effects is on replication, transcription, or translation.
HINT Each answer (replication, transcription, and translation) is used only once for the following:
a. Rifampin binds to bacterial RNA polymerase.
b. Streptomycin binds bacterial ribosomes, disabling them.
c. Quinolone blocks an enzyme that prevents bacterial DNA from unwinding.
Based on what you have learned with respect to various DNA repair pathways, decide the most appropriate pathway that would be used to repair the following types of DNA damage. Explain your reasoning.
A change in the DNA sequence caused by a mistake made by DNA polymerase during replication
In a fungal species, pyrimidine dimers induced as a result of UV exposure
A double-stranded break that occurs during G1 and prevents completion of DNA replication
Chapter 15 Solutions
Concepts of Genetics (12th Edition)
Ch. 15 - If a point mutation occurs within a human egg cell...Ch. 15 - One of the most famous cases of an X-linked...Ch. 15 - The cancer drug melphalan is an alkylating agent...Ch. 15 - Geneticists often use the alkylating agent...Ch. 15 - Six months pregnant, an expectant mother had a...Ch. 15 - Six months pregnant, an expectant mother had a...Ch. 15 - HOW DO WE KNOW? In this chapter, we focused on how...Ch. 15 - CONCEPT QUESTION Review the Chapter Concepts list...Ch. 15 - What is a spontaneous mutation, and why are...Ch. 15 - Prob. 4PDQ
Ch. 15 - Prob. 5PDQCh. 15 - Why is a random mutation more likely to be...Ch. 15 - Most mutations in a diploid organism are...Ch. 15 - What is the difference between a silent mutation...Ch. 15 - Describe a tautomeric shift and how it may lead to...Ch. 15 - Contrast and compare the mutagenic effects of...Ch. 15 - Why are frameshift mutations likely to be more...Ch. 15 - Why are X rays more potent mutagens than UV...Ch. 15 - DNA damage brought on by a variety of natural and...Ch. 15 - Contrast the various types of DNA repair...Ch. 15 - Mammography is an accurate screening technique for...Ch. 15 - A significant number of mutations in the HBB gene...Ch. 15 - Describe how the Ames test screens for potential...Ch. 15 - Prob. 18PDQCh. 15 - Compare DNA transposons and retrotransposons. What...Ch. 15 - Prob. 20PDQCh. 15 - In maize, a Ds or Ac transposon can alter the...Ch. 15 - Prob. 22PDQCh. 15 - In a bacterial culture in which all cells are...Ch. 15 - Presented here are hypothetical findings from...Ch. 15 - Prob. 25ESPCh. 15 - Prob. 26ESPCh. 15 - What evidence indicates that mutations in human...Ch. 15 - Among Betazoids in the world of Star Trek, the...Ch. 15 - Skin cancer carries a lifetime risk nearly equal...Ch. 15 - It has been noted that most transposons in humans...Ch. 15 - Mutations in the IL2RG gene cause approximately 30...
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- Adjacent pyrimidine bases in DNA form dimers with high efficiency after exposure to UV light. If these dimers are not repaired, skin cancers can result. Melanin is a natural sunscreen produced by melanocytes, a type of skin cell, when the skin is exposed to sunlight. Individuals who spend long periods developing a tan eventually acquire a thick and highly wrinkled skin. Such individuals are also at high risk for skin cancer. Can you explain, in general terms, why these phenomena are related?arrow_forwardEnediynes are natural products with potent antitumor properties because they are able to cleave DNA (page 288). Their cytotoxic properties are due tothe enediyne undergoing a cyclization to form a highly reactive diradical intermediate. The intermediate abstracts hydrogen atoms from the backbone of DNA, which triggers its damage. Draw the structure of the diradical intermediate.arrow_forwardChlorambucil is a nitrogen mustard. What is the structure of its covalent adduct to DNA?arrow_forward
- Emtricitabine (2',3'-dideoxy-5-fluoro-3'-thiacytidine, abbreviated as FTC) is a nucleoside analog that is used to treat HIV. It works by reversibly binding to HIV reverse transcriptase (HIV RT) and by doing so, inhibits the virus from replicating itself. In an experiment, FTC and purified HIV RT are mixed at low concentrations and allowed to reach equilibrium. The concentrations measured are [FTC] = 10 nM, [HIV RT] = 37.5 nM, and [HIV RT-FTC] = 2.5 µM, for the equilibrium FTC + HIV RT= HIV RT-FTC. What is the Kd in nM?arrow_forwardInsulin is a peptide hormone encoded by the INS gene, which is on chromosome 11. Insulin is produced by pancreatic beta cells. A) Both the INS gene and insulin are examples of biological molecules that are polymers. What are the monomers and what is the polymer that make up the INS gene? What is the name of the monomers and polymer that make up insulin? B). How many copies of the INS gene are found in pancreatic beta cells? How many copies are found in other cell types, for example, cardiac muscle cells? C). Is mRNA for insulin produced in equal amounts in pancreatic beta cells and cardiac muscle cells? Why or why not? D) Diabetes mellitus can be caused by a dominant mutation in the INS gene, INS*C96Y. A healthy mother has an affect child. Give the genotype of the child at the INS locus, indicating the paternally and maternally inherited alleles.arrow_forwardThe letters ABCDEFGH represent a normal DNA sequence. Indicate the type of mutation present in each of the following situations: a) ABCCDEFGH b) ABCDEGHarrow_forward
- Cytosine can be deaminated to form Uracil What type of mutation is this classified as? Discuss what happens to the base-pairing properties from switching from C to U? When U is replicated in two rounds of synthesis, what substitution does this result in? Before Uracil alters the DNA during replication, what repair system can be used to correct this error? Describe how this type of DNA repair works?arrow_forwardEthanol (CH3-CH2-OH) is miscible in water because it is able to form hydrogen bonds with itself and other molecules. However, its structure only allows it to form 1-2 hydrogen bonds. This is one reason why even low concentrations of ethanol in solution are lethal for cells. Based on this information, explain why we can use high concentrations of ethanol to precipitate DNA out of solution. Also, describe/predict the effects of increasing concentrations of ethanol in (and around) a cell on macro-molecular interactions (i.e. on weak bonds). Finally, it is possible to select for yeast that are tolerant to increased concentrations of ethanol. Give an example of a physiological change in yeast cells that might make them resistant to ethanol.arrow_forwardWhat entropic factor destabilizes helical DNA at high temperature?arrow_forward
- Hydrolysis of the N-glycosyl bond between deoxyribose and a purine base in DNA creates an apurinic (AP) site. An AP site is more thermodynamically destabilizing to a DNA molecule than is a mismatched base pair. Examine the structure of an AP site. H₂N HN N O™ -O-P-O-CH₂ Guanine H₂N N HN Select the chemical consequences that could contribute to DNA instability at AP sites. H H 1₂0/ H fewer hydrogen bonds between the unpaired pyrimidine base and water disruption of the base-stacking interactions decreased interaction between the mutated DNA strand and histones increased ability of the deoxyribose ring to open without the attachment of the purine base H H Guanosine residue (in DNA) O™ -O-P-O-CH₂ O H H H O Apurinic residue H OH Harrow_forwardGive the name of the enzyme that catalyzes each of the following reactions:(a) Makes a DNA strand from a DNA template. (b) Makes a DNA strand from an RNA template. (c) Makes an RNA strand from a DNA templatearrow_forwardThere are two types of excision repair, base excision repair (BER) and nucleotide excision repair (NER). Which enzymes are used in Base excision repair in E. coli?And In nucleotide excision repair, what kind of damage is repaired by this mechanism and why are cutsmade on both sides of the damaged region of DNA?arrow_forward
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