Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 14.5, Problem 1COMQ
For a riboswitch that controls transcription, the binding of a small molecule such as TPP controls whether the RNA
a. has an antiterminator or terminator stem-loop.
b. has a Shine-Dalgarno antisequestor or the Shine-Dalgarno sequence within a stem-loop.
c. is degraded from its 5′ end.
d. has an antiterminator or terminator stem-loop and has a Shine-Dalgarno antisequestor or the Shine-Dalgarno sequence within a stem-loop.
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If we watched a eukaryotic cell initiate translation, one of the first things we would see is
a.
The small ribosomal subunit binding to the cap
b.
The formation of the 70s initiation complex
c.
The small ribosomal subunit binding to the Shine-Dalgarno sequence
d.
The formation of the 80s initiation complex
Which sequence is most likely to be found in a promoter?
a) CGGTGTATATCGTAC
b) GTACAGTCATCCCGT
c) AAATCTACTACGATT
d) GGGTTGGGTTGGGTT
Gene expression in bacteria is controlled by the operon model.
a) Why is the operon model important?
b) Why is the trp operon known as a repressible operon?
Chapter 14 Solutions
Genetics: Analysis and Principles
Ch. 14.1 - 1. A repressor is a __________ that _________...Ch. 14.1 - 2. Which of the following combinations will cause...Ch. 14.2 - 1. What is an operon?
a. A site in the DNA where...Ch. 14.2 - The binding of _______ to lac repressor causes lac...Ch. 14.2 - Prob. 3COMQCh. 14.2 - How does exposing an E. coli cell to glucose...Ch. 14.3 - When tryptophan binds to trp repressor, this...Ch. 14.3 - During attenuation, when tryptophan levels are...Ch. 14.3 - Operons involved with the biosynthesis of...Ch. 14.4 - 1. Translation can be regulated by
a....
Ch. 14.4 - Prob. 2COMQCh. 14.5 - 1. For a riboswitch that controls transcription,...Ch. 14 - 1. What is the difference between a constitutive...Ch. 14 - In general, why is it important to regulate genes?...Ch. 14 - Prob. 3CONQCh. 14 - Transcriptional regulation often involves a...Ch. 14 - An operon is repressible-a small effector molecule...Ch. 14 - Prob. 6CONQCh. 14 - 7. What is enzyme adaptation? From a genetic point...Ch. 14 - Prob. 8CONQCh. 14 - Prob. 9CONQCh. 14 - Prob. 10CONQCh. 14 - Mutations may have an effect on the expression of...Ch. 14 - 12. Would a mutation that inactivated lac...Ch. 14 - Prob. 13CONQCh. 14 - 14. As shown in Figure 14.12, four regions within...Ch. 14 - 15. As described in Chapter 13, enzymes known as...Ch. 14 - 16. The combination of a 3–4 stem-loop and a...Ch. 14 - 17. Mutations in tRNA genes can create tRNAs that...Ch. 14 - Prob. 18CONQCh. 14 - Prob. 19CONQCh. 14 - A species of bacteria can synthesize the amino...Ch. 14 - 21. Using three examples, describe how allosteric...Ch. 14 - How are the actions of lac repressor and trp...Ch. 14 - 23. Transcriptional repressor proteins (e.g., lac...Ch. 14 - Answer the following questions that pertain to the...Ch. 14 - Chapter 21 describes a blotting method known as...Ch. 14 - As described in experimental question E2 and also...Ch. 14 - Prob. 4EQCh. 14 - Explain how the data shown in Figure 14.9 indicate...Ch. 14 - Prob. 6EQCh. 14 - Lets suppose you have isolated a mutant strain of...Ch. 14 - Prob. 1QSDCCh. 14 - Prob. 2QSDC
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- The following diagram represents a transcription unit on a DNA molecule. a. Assume that this DNA molecule is from a bacterial cell. Draw the approximate locations of the promoter and terminator for this transcription unit. b. Assume that this DNA molecule is from a eukaryotic cell. Draw the approximate location of an RNA polymerase II promoter.arrow_forwardf you made a change in the promoter sequence in the DNA that inactivates the promoter, what would happen at the RNA level? A-Nothing, because the RNA would be made as usual B-Transcription factors would be unable to bind and the RNA polymerase would not be recruited to the DNA, so no RNA would be made. C-The mutation of the DNA would be carried through to the RNA sequence. D-The DNA helicase would not be able to recognize and bind the DNA, so the RNA would not be made. EXPLAIN WHY THE ANSWER YOU CHOOSE IS CORRECTarrow_forwardIf a splice site were mutated so that splicing did not take place, what would be the effect on the mRNA? a. It would be shorter than normal. b. It would be longer than normal. c. It would be the same length, but would encode a different protein.arrow_forward
- a) what is a promoter and give the element and their functions of E.coli promoter b) what are eukaryotic transcription factor and list the class 2 general transcription factors and state their functionsarrow_forward. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?arrow_forwarda mutation in the gene encoding Dicer would most likely result in: A) a failure to break the H bonds holding the 2 strands of the siRNA together B) the inability to form H bonds betwen the siRNA and the complementary mRNA that it targets. C) the inability of the target mRNA to be cleaved upon H bonding with the siRNA D) a failure to produce siRNAs responsible for knocking down gene expression E) the failure to produce a dsRNA specific to your gene of interestarrow_forward
- If a mutation occurred in a control element sequence associated with a particular gene, which of the following would be a likely consequence of this? A. This mutation is not likely to affect the expression of the associated gene, because RNA polymerase does not bind to control elements. B. This will likely affect the expression of the associated gene because it will loosen the coiling of chromatin at this site. C. The expression of the associated gene will be affected because this change will result in a nonsense mutation. D. This mutation is not likely to affect the expression of the associated gene, since control elements are noncoding sequences. E. The associated gene's expression would likely be affected, since this mutation would change the ability of at least one transcription factor to bind.arrow_forwardTranscription in prokaryotes produces mRNA that is ready to translate, while in eukaryotes the RNA must be processed by... A. Adding a 5' cap, which is a version of a guanine nucleotide B. Adding a 3' poly A tail, which is a series of adenosines C. Removing introns D. A and B E. A, B, and Carrow_forwardIn the triplet-binding assay of Nirenberg and Leder, an RNA triplet composed of three bases was able to cause the a. translation of a polypeptide. b. binding of a tRNA carrying the appropriate amino acid. c. termination of translation. d. release of the amino acid from the tRNA.arrow_forward
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