The protocol of the experiment was to first grow the mutant and the merozygote strain separately into two conical flasks and then, the strains were divided into four tubes. Two of the tubes had mutant strains and the rest of the two hadmerozygote strain. To one of the merozygotes and mutant strain, lactose was added. The cells were incubated and then, sonicated to release enzymes. Then, o- nitrophenol was added and color of the solution in the tubes was measured using a spectrophotometer.

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Chapter 14, Problem 4EQ

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The protocol of the experiment was to first grow the mutant and the merozygote strain separately into two conical flasks and then, the strains were divided into four tubes. Two of the tubes had mutant strains and the rest of the two hadmerozygote strain. To one of the merozygotes and mutant strain, lactose was added. The cells were incubated and then, sonicated to release enzymes. Then, o-nitrophenol was added and color of the solution in the tubes was measured using a spectrophotometer.

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