Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Textbook Question
Chapter 12, Problem 4P
Identify similarities and differences between an inducible operon and a repressible operon in terms of
a. the transcription
b. the presence and action of allosteric regulatory molecules.
c. the organization of structural genes of the operon.
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Students have asked these similar questions
a. What is the function of operons in bacterial gene regulation?
b. Describe how a bacterial operon can be regulated by repressible proteins (such as the tryptophan operon). Include in your description both the “on” and “off” states of the operon. Key elements of your diagram should include: Co-repressor, Genes, mRNA, Operator, Operon, Promoter, Repressor, RNA polymerase.
B. A researcher was trying to determine whether two molecules (A and B) were corepressors or inducers in their respective
operon systems. Data was collected regarding the levels of protein and the amount of gene transcription for the genes in
their respective operons. The data is shown below:
Level of protein
High
Transcription of gene 1
Transcription of gene 2
Molecule A
Low
Low
High
High
low
High
High
Low
Molecule B
High
low
Low
a) Dete
b) Identify which system resembles the lac operon system and which resembles the trp operons system
nine whether molecule A and molecule B are
ducers or corepressors. Justify your answers.
1.
A)Explain the mechanism by which the lac operon is sensitive to glucose levels (presence and absence of glucose).
B)If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
Chapter 12 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 12 - 12.1 Bacterial genomes frequently contain groups...Ch. 12 - Transcriptional regulation of operon gene...Ch. 12 - Why is it essential that bacterial cells be able...Ch. 12 - Identify similarities and differences between an...Ch. 12 - The transcription of -galactosidase and permease...Ch. 12 - 12.6 Is attenuation the product of an allosteric...Ch. 12 - The trpL region contains four repeated DNA...Ch. 12 - The CAP binding site in the lac promoter is the...Ch. 12 - What role does cAMP play in transcription of lac...Ch. 12 - How would a cap- mutation that produces an...
Ch. 12 - Explain the circumstances under which attenuation...Ch. 12 - Consider the transcription of genes of the...Ch. 12 - Describe the lytic and lysogenic life cycles of ...Ch. 12 - 12.14 Define antisense RNA, and describe how it...Ch. 12 - 12.15 Attenuation of trp operon transcription is...Ch. 12 - 12.16 In the lac operon, what are the likely...Ch. 12 - Identify which of the following lac operon haploid...Ch. 12 - Prob. 18PCh. 12 - 12.19 List possible genotypes for lac operon...Ch. 12 - Suppose each of the genotypes you listed in parts...Ch. 12 - 12.21 Four independent mutants (mutants A to D)...Ch. 12 - Suppose the lac operon partial diploid...Ch. 12 - What is a riboswitch? Describe the riboswitch...Ch. 12 - 12.24 A repressible operon system, like the trp...Ch. 12 - 12.25 What is the likely effect of each of the...Ch. 12 - 12.26 Suppose that base substitution mutations...Ch. 12 - 12.27 Two different mutations affect. Mutant...Ch. 12 - How would mutations that inactivate each of the...Ch. 12 - The bacterial insertion sequence IS 10 uses...Ch. 12 - For an E. coli strain with the lac operongenotype...Ch. 12 - 12.31 How could antisense RNA be used as an...Ch. 12 - 12.32 Section describes the function of tRNA...Ch. 12 - The following hypothetical genotypes have genes A,...Ch. 12 - 12.34 Northern blot analysis is performed on...Ch. 12 - Prob. 35PCh. 12 - Prob. 36PCh. 12 - 12.37 The electrophoresis gel shown in part (a) is...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Tryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. c) Review the region that is responsible for trp attenuation during low trp level.arrow_forward. a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?arrow_forwardThe lactose operon in E. coli comprises two structural genes and three regulatory components.a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its functionarrow_forward
- 1) A. What is the difference between a repressible vs, an inducible operon? B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.arrow_forwardTryptophan (Trp) attenuation is an extra mechanism of regulation of trp operon when trp level is too high. Attenuation causes premature transcription termination. b) Predict what will happen if trp repressor lose its allosteric effect.arrow_forwardDefine the following in the context of operons: a. positive control b. give an example of an operon that is under positive control c. negative control d. give an example of an operon that is under negative control e. repressor f. give an example of a repressor molecule g. activator h. give an example of an activatorarrow_forward
- . If a particular operon encodes enzymes for makingan essential amino acid and is regulated like thetrp operon, then(A) the amino acid inactivates the repressor.(B) the repressor is active in the absence of theamino acid.(C) the amino acid acts as a corepressor.(D) the amino acid turns on transcription of the operonarrow_forwardIf glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forwardThe trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino acid tryptophan. (i) Draw a schematic diagram showing the components involved in regulation of the tryptophan operon. (ii) How does the regulation of the trp operon differ from the regulation of the lac operon? Explain.arrow_forward
- Control of the trp operon relies on which of the following: a. High levels of tryptophan causing a pause (but not termination) in transcription b. Low levels of tryptophan causing a pause (but not termination) in transcription c. Allolactose binding to the inhibitor to prevent it from binding to the promoter d. Tryptophan binding to the inhibitor to prevent it from binding to the promoterarrow_forwardHow will transcription of the E. coli trp (tryptophan) operon be affected by the following manipulations of the leader region of the trp mRNA?(a) Increasing the distance (number of bases) between the leader peptide gene and region 2(b) Increasing the distance between region 2 and region 3(c) Removing region 4(d) Changing several nucleotides in region 3 so that in can base pair with region 4 but not with region 2arrow_forwardWhat exactly is an “operon”? Briefly formulate your own definition of an operon. Include its function and cis-regulatory elements. Can you please explain in detailsarrow_forward
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