Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 12, Problem 16P
In the lac operon, what are the likely effects on operon gene transcription of the mutations described in a-e?
a. Mutation of consensus sequence in the lac promoter
b. Mutation of the repressor binding site on the operator sequence
c. Mutation of the lacI gene affecting the allosteric site of the protein
d. Mutation of the lacI gene affecting the DNA
e. Mutation of the CAP binding site of the lac promoter
Expert Solution & Answer
Trending nowThis is a popular solution!
Students have asked these similar questions
Consider the lac operon of E. coli. Specifically, explain the following:
a. Describe the overall chromosomal structure/organization of the operon, indicating the location
and function of the regulatory regions and the structural genes.
b. Is lac a catabolic or anabolic pathway? Explain. Is the operon inducible or repressible? Explain.
c. Describe the mechanisms involved in negative control & positive control
There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model,
a. How many ribosome binding sites are there for the protein?
b. How many promoters are there for the genes?
c. How many start codons are there for the protein?
d. How many RNA Polymerase binding locations are there for the genes?
e. How many proteins will be fully functional?
f. How many mRNA strands are made?
ate) DEFINE what is an Operon, and compare and contrast regulation of the LAC Operon and the TRP Operon.
When is each turned ON or OFF?
A. DRAW each operon in the PRESENCE of its own ligands (signal molecules).
B. What controls the activity of the regulatory proteins involved?
· Be sure to describe and show both POSITIVE and NEGATIVE regulation where appropriate.
C. Explain HOW each type of control system is appropriate for an operon encoding either catabolic or anabolic enzymes.
Chapter 12 Solutions
Genetic Analysis: An Integrated Approach (3rd Edition)
Ch. 12 - 12.1 Bacterial genomes frequently contain groups...Ch. 12 - Transcriptional regulation of operon gene...Ch. 12 - Why is it essential that bacterial cells be able...Ch. 12 - Identify similarities and differences between an...Ch. 12 - The transcription of -galactosidase and permease...Ch. 12 - 12.6 Is attenuation the product of an allosteric...Ch. 12 - The trpL region contains four repeated DNA...Ch. 12 - The CAP binding site in the lac promoter is the...Ch. 12 - What role does cAMP play in transcription of lac...Ch. 12 - How would a cap- mutation that produces an...
Ch. 12 - Explain the circumstances under which attenuation...Ch. 12 - Consider the transcription of genes of the...Ch. 12 - Describe the lytic and lysogenic life cycles of ...Ch. 12 - 12.14 Define antisense RNA, and describe how it...Ch. 12 - 12.15 Attenuation of trp operon transcription is...Ch. 12 - 12.16 In the lac operon, what are the likely...Ch. 12 - Identify which of the following lac operon haploid...Ch. 12 - Prob. 18PCh. 12 - 12.19 List possible genotypes for lac operon...Ch. 12 - Suppose each of the genotypes you listed in parts...Ch. 12 - 12.21 Four independent mutants (mutants A to D)...Ch. 12 - Suppose the lac operon partial diploid...Ch. 12 - What is a riboswitch? Describe the riboswitch...Ch. 12 - 12.24 A repressible operon system, like the trp...Ch. 12 - 12.25 What is the likely effect of each of the...Ch. 12 - 12.26 Suppose that base substitution mutations...Ch. 12 - 12.27 Two different mutations affect. Mutant...Ch. 12 - How would mutations that inactivate each of the...Ch. 12 - The bacterial insertion sequence IS 10 uses...Ch. 12 - For an E. coli strain with the lac operongenotype...Ch. 12 - 12.31 How could antisense RNA be used as an...Ch. 12 - 12.32 Section describes the function of tRNA...Ch. 12 - The following hypothetical genotypes have genes A,...Ch. 12 - 12.34 Northern blot analysis is performed on...Ch. 12 - Prob. 35PCh. 12 - Prob. 36PCh. 12 - 12.37 The electrophoresis gel shown in part (a) is...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- . a. How many ribosomes are required (at a minimum)for the translation of trpE and trpC from a singletranscript of the trp operon?b. How would you expect deletion of the two tryptophan codons in the RNA leader to affect theexpression of the trpE and trpC genes?arrow_forwardWhich of the following statements is true regarding transcriptional attenuation of the trp operon? a. The leader peptide encodes a protein that binds to the operator site b. Translation of the leader peptide stalls when tryptophan levels are high c. Stalling of the ribosome in region 1 of the leader peptide inhibits formation of the transcriptional terminator sequence d. This kind of mechanism can only occur in prokaryotes e. Both c. and d. are truearrow_forwardMutations may have an effect on the expression of the lac operon and the trp operon. Would the following mutations have a cis- or transeffect on the expression of the protein-encoding genes in the operon? A. A mutation in the operator site that prevents lac repressor from binding to it B. A mutation in the lacI gene that prevents lac repressor from binding to DNA C. A mutation in the trpL gene that prevents attenuationarrow_forward
- 2) Attenuation of the trp operon is controlled by the formation of stem-loop structures in the mRNA of the trpl region. The attenuation function can be disrupted by mutations that alter the sequence of the repeat DNA regions 1-4 and prevent formation of the RNA stem loops. Describe the most likely effects on attenuation of each of the following mutation under the conditions specified. a. Mutation in region 1 and low levels of tryptophan: b. Mutation in region 2 and low levels of tryptophan: Mutation in region 2 and moderate levels of tryptophan (low enough to release the repression but enough to allow the ribosome to NOT pause): с. d. Mutation in region 3 and low levels of tryptophan: е. Mutation in region 3 and moderate levels of tryptophan (low enough to release the repression but enough to allow the ribosome to NOT pause): f. Mutation in region 4 and low levels of tryptophan:arrow_forwardSix strains of E. coli (mutants 1-6) that had one of the following mutations affecting the lac operon were isolated. a. deletion of lacY b. lacO mutation c. missense mutation in lacZ d. inversion of the lac operon (but not an inversion of the lacl gene) e. superrepressor mutation f. inversion of lacZ, Y, and A but not lacl, P, O The entire lac operon (including the lacI gene and its promoter) from each of the six E. coli strains was cloned into a plasmid vector containing an ampicillin resistance gene. Each recombinant plasmid was transformed into each of the six strains to create merodiploids, and each was plated on ampicillin media in which lactose is the only carbon source. (Ampicillin was included to ensure maintenance of the plasmid.) Growth of the transformants is scored here (a “+" sign = growth, a "-" sign = no growth). Synthesis of ß-galactosidase and permease are required for growth on this medium. Results of this merodiploid analysis are shown on the table given above. Which…arrow_forwardIf CAP is bound to the Promoter of the Lac Operon and the repressor protein is not bound to the operator, which of the following is true: a.Glucose Levels are low & Lactose is absent b.Glucose is absent & Lactose is present c.cAMP is low & Lactose is Absent d.Glucose and Lactose are Present e.cAMP is low & Lactose is presentarrow_forward
- If lactose isn't present in the environment of an E. coli, what is the state of the lac operon? O a. It is "on" (high transcription) because will glucose will still be present, so the operon's products will be needed. O b. It is "on" (high transcription) because CAP will be bound to the promoter O c. It is "off" (no transcription) because RNA polymerase will be inactivated d. It is "off" (no transcription) and the repressor will be bound to the operatorarrow_forwardListed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? a. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR b. A mutation that changes the Trp codons in region 1 of the mRNA 5′ UTR into codons for alanine c. A mutation that creates a stop codon early in region 1 of the mRNA 5′ UTR d. Deletions in region 2 of the mRNA 5′ UTR e. Deletions in region 3 of the mRNA 5′ UTR f. Deletions in region 4 of the mRNA 5′ UTR g. Deletion of the string of adenine nucleotides that follows region 4 in the 5′ UTRarrow_forward4) Suppose you have an E. coli in which the gene that codes for the lac repressor protein has been mutated in such a way that the protein produced can bind to both lactose and DNA (at its specific binding site in the lac operon promoter) simultaneously. What effect, if any, would this have on regulation of expression of the lac operon? Briefly (1 sentence) explain why you would predict the effect or lack of effect this mutation would have.arrow_forward
- A mutation that inactivates transcription and translation from the regulatory gene of an inducible operon could result in: Group of answer choices A inactivation of RNA polymerase. B irreversible binding of the repressor to the operator. C three of these choices are correct D inhibition of transcription of the structural genes within the operon. E two of these choices are correct F transcription of the structural genes within that operon.arrow_forwardControl of the trp operon relies on which of the following: a. High levels of tryptophan causing a pause (but not termination) in transcription b. Low levels of tryptophan causing a pause (but not termination) in transcription c. Allolactose binding to the inhibitor to prevent it from binding to the promoter d. Tryptophan binding to the inhibitor to prevent it from binding to the promoterarrow_forwardIf the above gene is one of the three structural genes of the lac operon that codes for the protein/ enzyme responsible for breaking lactose into two molecules of simple sugars, what triggers the activation of this gene? a. Absence of Inhibitory protein b. Presence of lactose c. Absence of lactose d. Presence of Inhibitory protein e. Absence of Regulatory proteinarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
QCE Biology: Introduction to Gene Expression; Author: Atomi;https://www.youtube.com/watch?v=a7hydUtCIJk;License: Standard YouTube License, CC-BY