The lactose operon in E. coli comprises two structural genes and three regulatory components. a.State the name of each structural gene and describe its function: b.) State the name of each regulatory component and describe its function

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
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The lactose operon in E. coli comprises two structural genes and three regulatory components.
a.State the name of each structural gene and describe its function:

b.) State the name of each regulatory component and describe its function

operon were generated.
B-galactosidase and permease enzymes was assayed in bacteria in medium
containing either glycerol or lactose as carbon sources. In the table below indicate if activity
of the enzyme is detected using + sign, or not detected using a-sign.
B-galactosidase activity
Glycerol
Permease activity
Glycerol
Genotype
Lactose
Lactose
+ P+ O+ Z+ Y+
I+ P+ O+ Z+ Y+
+ P+ O+ Z- Y+
- P+ O+ Z+ Y-
-P+ O+ Z- Y+
I+ P+ OC Z+ Y-
C
moy
IS P+ O+ Z+ Y-
I+ P+ O+ Z- Y+d
IS P+ OC Z+ Y-
I+ P+ O+ Z- Y+
The + or- symbols shown in the genotypes designate normal function or no function (respectively) of
he elements associated. C indicates that element functions constitutively. S indicates that the protein
encoded has super affinity for binding.
B.
Transcribed Image Text:operon were generated. B-galactosidase and permease enzymes was assayed in bacteria in medium containing either glycerol or lactose as carbon sources. In the table below indicate if activity of the enzyme is detected using + sign, or not detected using a-sign. B-galactosidase activity Glycerol Permease activity Glycerol Genotype Lactose Lactose + P+ O+ Z+ Y+ I+ P+ O+ Z+ Y+ + P+ O+ Z- Y+ - P+ O+ Z+ Y- -P+ O+ Z- Y+ I+ P+ OC Z+ Y- C moy IS P+ O+ Z+ Y- I+ P+ O+ Z- Y+d IS P+ OC Z+ Y- I+ P+ O+ Z- Y+ The + or- symbols shown in the genotypes designate normal function or no function (respectively) of he elements associated. C indicates that element functions constitutively. S indicates that the protein encoded has super affinity for binding. B.
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