Chapter 12, Problem 15P

Interpretation Introduction

(a) Interpretation:

To determine the steady state kinetic analysis of the muscle isozyme by lactate which is present or not by the following inhibited/uninhibited enzymes:

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(b) Interpretation:

The reciprocal of Vmax for the uninhibited enzyme.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(c) Interpretation:

The line representing the data obtained in the presence of lactate acting as a competitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(d) Interpretation:

The line representing data obtained in the presence of lactate acting as a noncompetitive inhibitor with respect to the variable substrate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(e) Interpretation:

The reciprocal of K_M in the presence of lactate acting as a competitive inhibitor.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.

Interpretation Introduction

(f) Interpretation:

The most appropriate NADH concentration to use when determining K_M for pyruvate.

Introduction:

Lactate dehydrogenase enzyme is needed during the break down of glucose molecules to obtain energy from it. this enzyme is present in the cells of different organs of the body including, kidney, liver, heart, skeletal muscles, blood cells, lymph tissue, and pancreas.