Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 10, Problem 4RQ
Summary Introduction
To review:
The process by which tryptophan represses the tryptophan operon.
Introduction:
Tryptophan is an amino acid that is needed by Escherichia coli to build proteins and for its survival. Tryptophan is not available in the environment, so E.coli needs to synthesize its own with the help of five genes that constitute the trp operon. These genes include a promoter and an operator. The five genes of trp operon are transcribed by single messenger, that is, RNA (ribonucleic acid). Tryptophan is used as a building block for the synthesis of protein.
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How does the level of tryptophan alter transcription of the trp operon?
What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case!
A) Glucose is absent and lactose is present
B) Glucose is present and lactose is present
C) Glucose is present and lactose is absent
What is an operon? Explain an inducible operon.
Chapter 10 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 10.1 - Prob. 1TQCh. 10.2 - Prob. 1TQCh. 10.2 - Prob. 2TQCh. 10.2 - Prob. 3TQCh. 10.2 - Prob. 4TQCh. 10.2 - Prob. 5TQCh. 10.3 - Prob. 1TQCh. 10.3 - Prob. 2TQCh. 10.4 - Prob. 1TQCh. 10.5 - Prob. 1TQ
Ch. 10.5 - Prob. 2TQCh. 10.6 - Prob. 1TQCh. 10 - Prob. 1RQCh. 10 - Prob. 2RQCh. 10 - Prob. 3RQCh. 10 - Prob. 4RQCh. 10 - Prob. 5RQCh. 10 - Prob. 6RQCh. 10 - Prob. 7RQCh. 10 - Prob. 8RQCh. 10 - Prob. 9RQCh. 10 - Prob. 10RQCh. 10 - Prob. 11RQCh. 10 - Prob. 12RQCh. 10 - Prob. 13RQCh. 10 - Prob. 14RQCh. 10 - Prob. 1TQCh. 10 - Prob. 2TQCh. 10 - Prob. 3TQCh. 10 - Prob. 4TQ
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- Gene expression in bacteria is controlled by the operon model. a) Why is the operon model important? b) Why is the trp operon known as a repressible operon?arrow_forwardWhat is a negative inducible operon?arrow_forwardWhat would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forward
- What is the function of the Z gene of the Lac operon?arrow_forwardWhat is the phenotype of an E. coli strain with a mutation in the lac operator that allows the lac repressor to irreversibly bind the operator? Assume glucose is absent. 1S A) O Transcription of the operon is very high whether lactose is present or not. B) O Transcription of the operon is very low whether lactose is present or not. C) O Transcription of the operon is high in the presence of lactose, and low in its absence. D) O Transcription of the operon is low in the presence of lactose, and high in its absence.arrow_forwardIf a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would you expect? A) The three genes of the lac operon will be expressed normally. B) The repressor will not be able to bind to the operon. C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated. D) LacZ will not be transcribed and Beta galactosidase will not be produced.arrow_forward
- The attenuation mechanism that helps block expression of the tryptophan operon requires all of the following, except A) O MRNA transcript of the trp leader sequence B) Oaribosome stalled at the stop codon of the trp leader transcript C) O formation of an attenuator loop D) O low intracellular levels of tryptophanarrow_forwardHow does the lac operon help E. coli conserve energy and other resources?arrow_forwardHow does the tryptophan operan turn back on in gene regulation?arrow_forward
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