Concept explainers
To review:
The results of fusion of a promoter for an SOS response gene with the luciferase open reading frame.
Introduction:
The class of oxidative enzymes that produces bioluminescence is defined as luciferase. They have wide applications in the field of
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The reporter genes are
It can be used as a real-time biosensor for environmental stress response. RecA (recombination A) protein that is stimulated by the single-stranded DNA (deoxyribonucleic acid) is involved in the inactivation of the repressor (LexA) of SOS response genes, which induce the response. The fusion of the luciferase gene with a recA promoter will show an increase in the fluorescence after the irradiation by ultraviolet light by putting the culture into a spectrophotometer.
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Microbiology: An Evolving Science (Fourth Edition)
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- There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model, a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?arrow_forwardThe figure below shows RNA-Seq data (RED) for the D. melanogaster transformer (tra) gene obtained from both adult female and male fruit flies. The blue lines indicate the tra gene structure, with thicker lines indicating exons, and thin lines introns. The 5' end of the gene is on the left, and the 3' end of the gene is on the right. Based on these data, the most likely conclusion is: Males and females express identical isoforms of tra Males express more tra RNA than females The female isoform has fewer amino acids The female isoform has more amino acids The male and female isoforms have different 3'UTRsarrow_forwardSuppose that the segment of the hsp70 gene that was used to make the hsp70/GFP fusion in the preceding problem had mutations in each of its heat-shock response elements. Would the green fluorescent protein encoded by this fusion gene be synthesized in genetically transformed flies?arrow_forward
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