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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
The transcription of many bacterial genes relies on
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- Gene expression in bacteria is controlled by the operon model. a) Why is the operon model important? b) Why is the trp operon known as a repressible operon?What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case! A) Glucose is absent and lactose is present B) Glucose is present and lactose is present C) Glucose is present and lactose is absentWhat is the phenotype of an E. coli strain with a mutation in the lac operator that allows the lac repressor to irreversibly bind the operator? Assume glucose is absent. 1S A) O Transcription of the operon is very high whether lactose is present or not. B) O Transcription of the operon is very low whether lactose is present or not. C) O Transcription of the operon is high in the presence of lactose, and low in its absence. D) O Transcription of the operon is low in the presence of lactose, and high in its absence.
- If a researcher moves the promoter for the lac operon to the region between the beta galactosidase (lacZ) gene and the permease (lacY) gene, which of the following results would you expect? A) The three genes of the lac operon will be expressed normally. B) The repressor will not be able to bind to the operon. C) The operon will still transcribe the lacZ and lacY genes, but the mRNA will not be translated. D) LacZ will not be transcribed and Beta galactosidase will not be produced.The attenuation mechanism that helps block expression of the tryptophan operon requires all of the following, except A) O MRNA transcript of the trp leader sequence B) Oaribosome stalled at the stop codon of the trp leader transcript C) O formation of an attenuator loop D) O low intracellular levels of tryptophanThe lac operon in E. coli bacteria codes for proteins that break down (digest) the disaccharide lactose. Therefore, the lac operon is expressed at its highest levels when there is: A) glucose but no lactose B) lactose and glucose C) lactose but no glucose D) no glucose or lactose
- What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)Describe operons in your own words, as if you were explaining what an operon is to someone who knew about DNA and cells etc but did not know what an operon was. Here are some things you can discuss: Are operons controlling transcription or translation? Which organisms are they found in? What do the genes in an operon have in common with each other?High levels of transcription of the lactose operon require all of the following, EXCEPT: A) O the presence of lactose B) O high levels of CAMP (cyclic AMP) C) O the absence of glucose D) O the formation of allolactose E) O A-D are all required
- Which of the following statements about the trp operon is false? If a mutation in region 2 prevents the formation of the 2 + 3 loop in the 5’UTR transcription levels will increase. The trp operon is an example of negative repressible operon. Formation of the anti-terminator in the 5’UTR of the trp operon occurs when trp levels are low. In the presence of high trp-tRNA, the genes of the trp operon are not expressed. The role of trp is to bind to the repressor protein and activate it.As diagrammed below, some amino acid biosynthetic operons have stem loop structures in the mRNA. These structures play a role in the regulation of the operon by which of the following mechanisms? Translation stop codon- Attenuator loop. mRNA 5' 3' DNA attenuator trpE O Catabolite repressor protein can bind and prevent the hairpins from forming thus allowing translation to occur. O The leader sequence shown contains codons for the amino acid. When these are low, the ribosome stalls, allowing a hairpin between the two middle regions to form so that the rest of the message can be translated. In the presence of large amounts of the amino acid, the hairpin will wind around the amino acid and prevent further translation. O A repressor protein made of that amino acid will bind to the region and prevent translation when large amounts of the amino acid are present. O The uridine-rich region has codons for that amino acid; when present, this region will be a terminator region. The hairpins allow…The lac operon contains the genes that the bacterium needs to be able to use lactose as a carbon source. What would happen to the regulation of the Lac operon if: a) a mutation has occurred in the Lac Z gene which results in the formation of no functional β-galactosidase b) a mutation has occurred in the CAP protein so that cAMP cannot bind