Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 10.2, Problem 4TQ
Summary Introduction
To review:
The preferred use of IPTG (isopropyl β-D-thiogalactopyranoside) in order to induce lacZYA operon.
Introduction:
IPTG is a chemical reagent with a similar structure to lactose. It is used in the induction of transcription in lac operon and further induction of protein expression at high levels. Catabolic repression is a phenomenon in which repression of lac operon occurs because glucose acts as a preferred carbon source instead of lactose and thus, glucose directly induces transcription.
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Students have asked these similar questions
Give all possible genotypes of a lac operon that produces, or fails to produce, β-galactosidase and permease under the following conditions. Do not give partial-diploid genotypes. Lactose absent Lactose present β-Galactosidase Permease β-Galactosidase Permease a. − − + + b. − − − + c. − − + − d. + + + + e. − − − − f. + − + − g. − + − +
Which of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose?
Group of answer choices
I+ O+ Z+ Y+ A+
I- O+ Z- Y- A-
I+ OC Z+ Y+ A+
IS O+ Z+ Y+ A+
I+ O+ Z- Y+ A+
The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene
operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this
operon, answer the following questions. Write numeric answers only. For example, if your
answer is 6 promoters, write only 6.
1) How many promoters control the expression of these genes?
2) How many locations does RNA Polymerase bind to get full expression of these genes?
3) How many ribosome binding sites are needed for full protein expression?
4) How many start codons will be needed for full protein expression?
5) How many mRNA strands will be produced with full operon expression?
6) How many proteins will be produced with full protein expression?
1
Chapter 10 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 10.1 - Prob. 1TQCh. 10.2 - Prob. 1TQCh. 10.2 - Prob. 2TQCh. 10.2 - Prob. 3TQCh. 10.2 - Prob. 4TQCh. 10.2 - Prob. 5TQCh. 10.3 - Prob. 1TQCh. 10.3 - Prob. 2TQCh. 10.4 - Prob. 1TQCh. 10.5 - Prob. 1TQ
Ch. 10.5 - Prob. 2TQCh. 10.6 - Prob. 1TQCh. 10 - Prob. 1RQCh. 10 - Prob. 2RQCh. 10 - Prob. 3RQCh. 10 - Prob. 4RQCh. 10 - Prob. 5RQCh. 10 - Prob. 6RQCh. 10 - Prob. 7RQCh. 10 - Prob. 8RQCh. 10 - Prob. 9RQCh. 10 - Prob. 10RQCh. 10 - Prob. 11RQCh. 10 - Prob. 12RQCh. 10 - Prob. 13RQCh. 10 - Prob. 14RQCh. 10 - Prob. 1TQCh. 10 - Prob. 2TQCh. 10 - Prob. 3TQCh. 10 - Prob. 4TQ
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- IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose. Based on your knowledge of the regulation of the lac operon, predict with explanation whether these gene products (B-galactosidase, lactose Permease) will be produced. a IPOZY IPOZY b. IP'O'Z'Y/IPOZY" +IPTG B-galactosidase -IPTG Permease B-galactosidase Permease Only typed answer. Will upvote you immediately. Please helparrow_forwardWhat would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)arrow_forwardWhat would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forward
- Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein. Otherwise all other parts of the operon are functional. 1. Can strain P77 metabolize lactose if it is present? 2. If lactose is absent, will strain P77 transcribe its lac operon? 3. If lactose is present, will strain P77 transcribe its lac operon?arrow_forwardLet’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation mayblock the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operator in a way that prevents lac repressor from binding to the operator. How would you distinguish between these two possibilities?arrow_forwardLet’s suppose you have isolated a mutant strain of E. coli in which the lac operon is constitutively expressed. In other words, the operon is turned on in the presence or absence of lactose. One possibility is that the mutation may block the transcription of the lacI gene, thereby preventing the synthesis of lac repressor. A second possibility is that the mutation could alter the sequence of the lac operon in a way that prevents the repressor protein from binding to the operator. How would you distinguish between these two possibilities?arrow_forward
- To study the lac operon, you engineer a strain of E coli to have a lac operon in which the lac Z gene is replaced by the gene for green fluorescent protein (GFP). Expression of GFP generates a green color in the cells that can be easily quantitated with a fluorescence microscope. You test the activity of the operon in the absence of the inducer IPTG, the presence of the inducer IPTG and the presence of an antibiotic the completely inhibits RNA polymerase (i.e. no gene expression). You then use this system to test the effects of various mutation on the activity of the operon. Match the following mutations with the activity (A, B or C) you would expect to observe with the mutation. All experiments are done in the presence of IPTG unless otherwise stated.arrow_forwardYou have an E.coli strain in which the lac operon is not expressed when glucose is absent and lactose is present. You have already conducted tests with partial diploids to rule out lacl, lacO, lacZ, lacY and lacA as the culprits, so you know that something must be wrong with the positive regulation system. Which of the following mutations could be causing this mutant phenotype? O cya- where glucose cannot bind adenylate cyclase O cap- where CAMP cannot bind to CAP M cap- where CAP cannot bind to the CAP binding site O Mutation in the CAP binding site that prevents CAP from binding O cya- where adenylate cyclase cannot turn ATP into CAMParrow_forwardStrain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind allolactose. Otherwise all other parts of the operon are functional. 1. Can strain LOLZ metabolize lactose if it is present? 2. If lactose is absent, will strain LOLZ transcribe its lac operon? 3. If lactose is present, will strain LOLZ transcribe its lac operon?arrow_forward
- Suppose you are studying the regulation of a gene involved in the metabolism of two nutrients, Llamasin and Alpacalon, in bacteria. You are trying to determine if these nutrients act as inducers in their operons. The following data were collected from your experiments. Which of these operons is most similar to the lac operon? Explain. Nutrient Levels of nutrient in growth medium Level of transcription of genes in operon Llamasin low high high low Alpacalon low low high high Highlight one in green: Llamasin Alpacalon Explanation:arrow_forwardDescribe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.arrow_forwardWhat experimental results would indicate that the mutation lacISlacIS is dominant to lacI+lacI+? In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a constitutive state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in an activated state in the presence of lactose. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the absence of the repressor. In lacISlacIS/lacI+lacI+ partial diploids, the lac operon is in a repressed state in the presence of lactose.arrow_forward
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