Substrate, uM VO, uM/min 2.5 28 4 40 10 70 20 95 40 112 100 128 200 139 1000 140
Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter2: Basic Hematology
Section2.13: Erythrocyte Sedimentation Rate
Problem 2CS
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Below is kinetic data obtained for an enzyme-catalyzed reaction. The enzyme concentration is fixed at 100 nM.
Using a Lineweaver-Burke plot, calculate the Km value for this reaction. Report your answer to three significant figures in units of uM.
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