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Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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A Plasmid is 3.6kb large. It is digested with an enzyme that has restriction sites at 1,152 and 2,954. How many fragments do we have and what are their sizes?
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- How many fragments and how big each fragment should be after digesting the plasmid with BamHI? How many fragments and how big each fragment should be after digesting the plasmid with PvuI? How many fragments and how big each fragment should be after digesting with both enzymes? Show your calculation/explanation.arrow_forwardDNA is extracted from the blood cells of two different humans, individuals 1 and 2. In separate experiments, the DNA from each individual is cleaved by restriction endonucleases A, B, and C, and the fragments are separated by electrophoresis. A hypothetical map of a 10,000 bp (base pair) segment of a human chromosome is shown (1 kbp= 1,000 bp). Individual 2 has point mutations that eliminate restriction recognition sites B* and C*. After the 10kbp segment is digested with each restriction endonuclease A, B, and C one by one, samples are loaded onto an agarose gel for electrophoresis for analysis. I would like you to draw the result of gel electrophoresis. To answer the question, first, draw the whole gel image (on the right) on your answer sheet, then indicate where you expect to see the bands on the gel. (Hint1: Same-size bands will appear at the same position. Hint 2: On the gel bands from individuals 1 and 2 might be at different positions.) The left lane (with an M label on the…arrow_forwardFor a restriction enzyme that recognizes the restriction site GGCC, Which of the following statements is/are true?arrow_forward
- A 2.0kb bacterial plasmid ‘BS1030’ is digested with the restriction endonuclease Sau3A; the plasmid map is depicted in the diagram below and the Sau3A (S) restriction sites are indicated. Which of the following DNA fragments do you expect to see on an agarose gel when you run Sau3A-digested plasmid ‘BS1030’ DNA? a. 250 bp, 450 bp, 550 bp, 1.1 kb, 1.5 kb and 2.0 kb b. 2.0kb c. 250 bp, 400 bp, 450 bp, 500 bp and 550 bp d. 100 bp, 200 bp, 250 bp, 400 bp, 500 bp and 550 bparrow_forwardAssume that a plasmid is 4700 base pairs in length and has restriction sites for a given restriction enzyme at the following locations: 800, 1400, 2900, and 3600. List the fragments by size that are ! expected when the plasmid is fully digested the restriction enzyme.arrow_forwardWhat is the size for cut lane #5 to measure the Sac1? The T-vector size is 3kb, what is the size for the insert?arrow_forward
- Match the following Conjugation terms with the most appropriate description in the image: F- recombinant F- strain F+ strain HFR strainarrow_forwardYou isolated two bacteria from soil that you assumed were different species since they have different properties - for example, one is resistant to several antibiotics and the other is not. However, upon sequencing the 16S rRNA genes of the isolates, you have discovered that they are the same genus and species. When you extract the total DNA from each and run a gel, you discover the resistant strain has a plasmid in addition to the primary Chromosome. a. How might this strain have acquired the plasmid? b. What is definitely encoded on the plasmid given the information provided? c. How could you use fluorescence microscopy to determine if other bacteria in your soil suspension are carrying the same plasmid? Be sure to comment on the type of fluorescent probe, how it will interact with your sample, and how you will distinguish cells harboring the plasmid from the rest of the population (what will it look like).arrow_forwardClone number in this case is number 196 as shown in the images. What is the exact length of the segment of human DNA that has been inserted into the plasmid? *report the entire length of the insert, not just the sequences matching the ends and labels of wells isn't needed for answer*arrow_forward
- Give a clear handwritten answer...?arrow_forwardBelow is shown a plasmid map from a recombinant DNA experiment you have performed. You inserted your gene of interest (labeled gene on the map) into the lac Z reporter gene via a Hind III site. If you digested this recombinant DNA with HindIII what is the size of the DNA fragment(s) you would obtain on a DNA gel electrophoresis? The plasmid alone is 4000 bp. I have attached the image below.arrow_forwardE 1 kb 1 kb DAK 6 kb Gene X E1 kb B 5 kb. Gene Z E₁kb 1 kb The figure above shows a linear chronosome containing Gene X and Gene Z and the location of sites for the restriction enzymes EcoRI (E) and BamHI (B). Refer to this map as you answer the question below. If you digest this DNA with EcoRI, then perform agarose gel electrophoresis, how many bands, and what sizes, would you expect to see? a) Two bands on the gel corresponding to the sizes 2 kb and 6 kb b) Three bands on the gel corresponding to the sizes 1 kb, 6 kb and 8 kb. c) Three bands on the gel corresponding to the sizes 1 kb, 5 kb and 6 kb d) Four bands on the gel corresponding to the sizes 1 kb, 2 kb, 5 kb and 6 kb e) Four bands on the gel corresponding to the sizes 2 kb, 3 kb, 4 kb and 8 kbarrow_forward
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