You isolated two bacteria from soil that you assumed were different species since they have different properties - for example, one is resistant to several antibiotics and the other is not. However, upon sequencing the 16S rRNA genes of the isolates, you have discovered that they are the same genus and species. When you extract the total DNA from each and run a gel, you discover the resistant strain has a plasmid in addition to the primary Chromosome. a. How might this strain have acquired the plasmid? b. What is definitely encoded on the plasmid given the information provided? c. How could you use fluorescence microscopy to determine if other bacteria in your soil suspension are carrying the same plasmid? Be sure to comment on the type of fluorescent probe, how it will interact with your sample, and how you will distinguish cells harboring the plasmid from the rest of the population (what will it look like).

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
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You isolated two bacteria from soil that you assumed were different species since they have different properties - for example, one is resistant to several antibiotics and the other is not. However, upon sequencing the 16S rRNA genes of the isolates, you have discovered that they are the same genus and species. When you extract the total DNA from each and run a gel, you discover the resistant strain has a plasmid in addition to the primary Chromosome.

a. How might this strain have acquired the plasmid?

b. What is definitely encoded on the plasmid given the information provided?

c. How could you use fluorescence microscopy to determine if other bacteria in your soil suspension are carrying the same plasmid? Be sure to comment on the type of fluorescent probe, how it will interact with your sample, and how you will distinguish cells harboring the plasmid from the rest of the population (what will it look like).

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