Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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- 32)An origin of replication is given below. Sequences of selected parental strand regions are given. The directionality of the bottom parental strand is indicated. Use the diagram to answer the corresponding questions. #2 *1 CTAAGCA ATCGAGG XXXXX XXXX 3' ICTAGTT 5' ge exon #3 a.) On the diagram, label the 5' and 3' end of the top strand of parental DNA b Draw arrows to indicate direction of DNA synthesis for each of the 4 daughter strands. e) For each daughter strand, specify if synthesis is continuous or discontinuous. d) On your diagram, label the 5' and 3' ends of the newly synthesized daughter strands e.) Which parental strands are the template for leading strand synthesis? (# 1- 4) Strand # Strand # f.) Which parental strands are the template for lagging strand synthesis? (# 1- 4) Strand # Strand # g.) What is the specific sequence of the primer required to start DNA replication ior strands 1 and 3? Assume the primer will bind to the location where the base sequence is given on…arrow_forwardA drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug? PICK ONE ANSWER: A. Keeping parent DNA strands separated B. Releasing tension generated by supercoiling C. Activating the origin of replication D. Making primers E. Forming phosphodiester bondsarrow_forwardWhich of the following enzymes is NOT involved in DNA replication? a. DNA Polymerase b. Primase c. Helicase d. Phosphatase e. DNA Ligasearrow_forward
- The following is a figure that depicts telomerase extending a telomere. -aaucccaauc ttag telomere The lagging strand template for DNA replication of this section of the chromosome is: a. Strand A Ob. Cannot be determined from the information given above O C. Not needed due to telomerase activity O d. Strand B O e. Strand Carrow_forward1. Cytosine deamination, in which cytosine is mutated to uracil, occurs spontaneously in DNA at a rate of 1 out of 107 cytosines in 24 hours. tofo NH₂ 'N cytosine deamination ----ဝိ NH spr a. If cytosine deamination occurs and DNA polymerase replicates the DNA with this altered base, what mutation would be introduced into DNA? b. Cytosine deamination is so common that DNA repair enzymes recognize uracil and replace it with cytosine. Given this information, why is it essential for DNA, the carrier of the genetic blueprint, to utilize thymine, not uracil, as a base?arrow_forward2b) Suppose you generated a mutant E. coli strain in which it was DNA Polymerase I that was inactivated - assuming that all the other enzymes involved in replication remained fully functional, how would DNA replication in these mutant cells lacking DNA Pol I differ from DNA replication in normal E. coli? Briefly explain why you would expect to see that change/those changes in DNA replication in the mutant cells.arrow_forward
- catalyzes the cleavage of DNA at a specific ii fragments. The statement given above is completed nucleotide sequence. splices DNA into by the information in row Row i ii A. DNA polymerase DNA ligase B. restriction DNA ligase endonuclease DNA Ligase restriction endonuclease restriction endonuclease С. D. restriction endonuclease A OB C Darrow_forwardSemiconservative replication of DNA means that: a. both daughter duplexes will be entirely new and the parental duplex will be degraded b. each daughter duplex will have one of the original parental strands and one new strand c. each strand of the daughter duplexes will have parts of the parental strand and parts of the new strand d. one daughter duplex will be entirely new and the other will have both original parental strandsarrow_forwardA DNA antisense strand contains the following nucleotide base sequence: ATC CAA GAC TGG From this, what is the nucleotide sequence of the MRNA strand that is transcribed? a. ATC CAA GÁC TGG b. TAG GTT CTG ACC c. UAG GUU CUG ACC d. AUC CAA GÁC UGGarrow_forward
- A student mixes various molecule needed for DNA replication. When he adds DNA, replication occurs, but each DNA duplex consists of a normal DNA strand paired with numerous segments of DNA a few hundred nucleotides long. What has been left out of the mixture? A. NTPs B. DNA polymerase III C. ATP D. DNA polymerase I E. DNA ligasearrow_forwardA mistake in DNA replication, in which the wrong base is placed in the growing DNA strand, can have profound effects on the descendents of any cells that carry the mistake.In the following strand, the A has been changed to a G. Draw the DNA molecules that result from replication of the altered strands. Original Strand T A G C G T T Mutated Strand T G G C G T T A T C G C A A A T C G C A Aarrow_forwardWhich of the following is NOT common to all repair mechanisms? A. Detection of the lesion B. Removal of the damaged DNA sequence/ nucleotide/ base C. Removal of free radicals at the site of injury D. Repair of the lesion E. Involvement of enzymes in removing the lesion and/or repair of the damaged sequence/nucleotide/base.arrow_forward
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