The arrow in the diagram below indicates the direction of movement of the replication fork. 5' A C B Replication Which letter indicates a strand that is synthesized discontinuously? OA B C 13' 35

Explain and demonstrate the steps in DNA replication using a replication fork.  Replicate 10 nucleotides onto the leading strand and 10 nucleotides on the lagging strand.

Which enzyme is responsible for proofreading nucleotides during DNA replication? a nuclease b exonuclease c ligase d polymerase

32)An origin of replication is given below. Sequences of selected parental strand regions are given. The directionality of the bottom parental strand is indicated. Use the diagram to answer the corresponding questions. #2 *1 CTAAGCA ATCGAGG XXXXX XXXX 3' ICTAGTT 5' ge exon #3 a.) On the diagram, label the 5' and 3' end of the top strand of parental DNA b Draw arrows to indicate direction of DNA synthesis for each of the 4 daughter strands. e) For each daughter strand, specify if synthesis is continuous or discontinuous. d) On your diagram, label the 5' and 3' ends of the newly synthesized daughter strands e.) Which parental strands are the template for leading strand synthesis? (# 1- 4) Strand # Strand # f.) Which parental strands are the template for lagging strand synthesis? (# 1- 4) Strand # Strand # g.) What is the specific sequence of the primer required to start DNA replication ior strands 1 and 3? Assume the primer will bind to the location where the base sequence is given on…