6. The following diagram shows a replication bubble within a double stranded DNA molecule. The red arrow shows the direction of the primer. мастасоттоло CATOCAACO 5' GTCCGAC 3' CAGGCAG ATTGGCC 3' TAACCGG 5' a. What is the sequence of the red RNA primer? b. If the replication fork moves to the left (left side opens more), will this primer be used to create the leading strand or lagging strand? Please explain your answer. c. If you answered the leading strand, explain why replication is continuous. However, if you answered lagging strand explain why replication is discontinuous.

33.Choose the DNA nucleotide sequence that would be complementary to the following strand (note: pay attention to DIRECTION of replication): 5' GTAGTCA 3' a 3' UATUAGA 5' b. 5' TGACTAC 3' 3' ACGACTG 5' 5' UGACUAC 3'. 5.Using agarose gel electrophoresis to analyze DNA, short DNA travels the long DNA. a faster than slower than at the same speed as d. Question 6 Which type of bonds link the individual nucleotides together in DNA? Glycosidic bond Peptide bond Phosphodiester bond Electrostatic bond a e Hydrogen bond Question 7 A typical engineered plasmid vector often contains the following features except a A poly linker region Origin of replication A signal peptide An antibiotic resistant gene a. b. C. C. d.

5. DNA is made of two strands that are antiparallel. If one strand runs from 3' to 5' direction the other one will go from 5' to 3' direction. During replication or transcription, whatever the process is, it will always follow the 5' to 3' direction using the 3' to 5' directed strand as the template strand. Therefore, if following is the DNA sequence 5'-CCG ATC GCA CAA-3' a) Using this sequence as template after transcription no protein can be translated. Why? I. Presence of start codon II. Absence of start codon III. Due to mutation b) If you want to start the translation, what change you need in the second codon (from 5' to 3' direction)? I. Substitution of C with G II. No change III. Deletion of C IV. Both I & III

1) Fill in the following replication bubble. Be sure to draw and label the origin of replication (both sides), the leading and lagging strands, & the 5' and 3' ends of alI DNA fragments. 5' 3'

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This is an absolute stumper. Would you be able to assist with drawing and labeling would look like in this scenario?

12. Shown below are three possible mechanisms for DNA replication. Identify which each kind is (semiconservative, conservative, or dispersive). I Which model is this? (select one) A. Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive Semiconservative Conservative Dispersive B. C. XXX< XXXXXX XXX< x< XXXXXXXX XXXXXXXxXxXx XXXXXX MXXXXX XXxxxxxX XXXXXX

Answer A, B, C all of them

1. Name all the proteins that are in the DNA replication fork in E. Coli, and describe the functions of these proteins. Explain how two DNA strands are replicated at the same time by one replication fork.

1. From the given DNA strands:a. Identify the template and non-template strand.b. Give the mRNA product when the DNA is transcribed.c. What is the resulting amino acid sequence which will result from the mRNA?