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Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. R CD4+ T cells transcriptional start site -PCR CD4+ T cells +1 -PCR CD8+ T cells AIG e markers intron V The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. intron transcriptional stop site Į TAA

Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. R CD4+ T cells transcriptional start site -PCR CD4+ T cells +1 -PCR CD8+ T cells AIG e markers intron V The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. intron transcriptional stop site Į TAA

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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If there is a three base pair insertion into the cxx3 gene in the location marked by the white
arrowhead, what difference (if any) would you expect to see in Cxx3 protein produced by CD4+ T
cells with this variant gene? Explain. 

Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. PCR CD4+ T cells transcriptional start site RT-PCR CD4+ T cells +1 RT-PCR CD8+ T cells AIG Size markers The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. wells +. intron DNA migration V intron transcriptional stop site Į TAA
Transcribed Image Text:Here is a second human gene, called cxx3. The structure indicates the following: the start and end of transcription, the start codon and the stop codon, the location of the two introns (marked with black boxes). The white boxes indicate the binding locations of two PCR primers. PCR CD4+ T cells transcriptional start site RT-PCR CD4+ T cells +1 RT-PCR CD8+ T cells AIG Size markers The researchers carry out a FACS (Fluorescence Activated Cell Sorting) separation of human blood which isolates a relatively pure sample of CD4+ T cells, and a relatively pure sample of CD8+ T cells, both different types of lymphocytes. They carry out a PCR, and a semi-quantitative RT-PCR, on these cell samples. The results are shown on the gel. wells +. intron DNA migration V intron transcriptional stop site Į TAA
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