FIGURE 21.13 The use of CRISPR-Cas technology to cause tracrRNA a gene mutation. (a) Structure of an sgRNA, which is composed of a crRNA that is connected to a tracrRNA via a linker. (b) Use of CRISPR- Cas technology. On the left side, the target gene has been repaired by NHEJ and has incurred a small deletion. This may result in gene inacti- vation. On the right side, the target gene has been repaired by HRR and now carries a point mutation. 3' Linker 5' Spacer RNA-complementary Repeat CONCEPT CHECK: How is the sgRNA different from certain components of the bacterial defense system described in Chapter 17 (see Figure 17.7)? to target gene crRNA (a) Structure of an sgRNA Region of target gene that is complementary to spacer RNA - SGRNA Cas9 Target gene The spacer region of the sgRNA binds to a complementary region of the target gene. Cas9 cleaves the target gene in both strands thereby generating a double-strand break. Cleavage Cleavage Nonhomologous end joining Homologous recombination repair Small deletion in target gene - Donor DNA Point mutation in donor DNA A double crossover swaps a portion of the target gene with the donor DNA. Point mutation now in target gene (b) Use of CRISPR-Cas technology to inactivate a gene or create a point mutation
FIGURE 21.13 The use of CRISPR-Cas technology to cause tracrRNA a gene mutation. (a) Structure of an sgRNA, which is composed of a crRNA that is connected to a tracrRNA via a linker. (b) Use of CRISPR- Cas technology. On the left side, the target gene has been repaired by NHEJ and has incurred a small deletion. This may result in gene inacti- vation. On the right side, the target gene has been repaired by HRR and now carries a point mutation. 3' Linker 5' Spacer RNA-complementary Repeat CONCEPT CHECK: How is the sgRNA different from certain components of the bacterial defense system described in Chapter 17 (see Figure 17.7)? to target gene crRNA (a) Structure of an sgRNA Region of target gene that is complementary to spacer RNA - SGRNA Cas9 Target gene The spacer region of the sgRNA binds to a complementary region of the target gene. Cas9 cleaves the target gene in both strands thereby generating a double-strand break. Cleavage Cleavage Nonhomologous end joining Homologous recombination repair Small deletion in target gene - Donor DNA Point mutation in donor DNA A double crossover swaps a portion of the target gene with the donor DNA. Point mutation now in target gene (b) Use of CRISPR-Cas technology to inactivate a gene or create a point mutation
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
How is the sgRNA different from certain components of the bacterial defense system described?
Transcribed Image Text:FIGURE 21.13 The use of CRISPR-Cas technology to cause
tracrRNA
a gene mutation. (a) Structure of an sgRNA, which is composed of a
crRNA that is connected to a tracrRNA via a linker. (b) Use of CRISPR-
Cas technology. On the left side, the target gene has been repaired by
NHEJ and has incurred a small deletion. This may result in gene inacti-
vation. On the right side, the target gene has been repaired by HRR and
now carries a point mutation.
3'
Linker
5'
Spacer RNA-complementary Repeat
CONCEPT CHECK: How is the sgRNA different from certain components of
the bacterial defense system described in Chapter 17 (see Figure 17.7)?
to target gene
crRNA
(a) Structure of an sgRNA
Region of target gene that is
complementary to spacer RNA
- SGRNA
Cas9
Target gene
The spacer region of the sgRNA binds to a complementary
region of the target gene. Cas9 cleaves the target gene in
both strands thereby generating a double-strand break.
Transcribed Image Text:Cleavage
Cleavage
Nonhomologous
end joining
Homologous
recombination repair
Small deletion
in target gene
- Donor DNA
Point mutation in donor DNA
A double crossover swaps
a portion of the target gene
with the donor DNA.
Point mutation now in target gene
(b) Use of CRISPR-Cas technology to inactivate a gene or create a point mutation
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