- Oligonucleotide primer with a mismatch Gene in -Mismatch a vector (template DNA) Vector Add DNTPS, DNA polymerase, and DNA ligase. -Mismatch The DNA is introduced into a living cell, where the mismatch is repaired. GG.C CCG CTS or A site-directed mutant is made. The DNA is repaired back to the original sequence. FIGURE 21.12 The method of site-directed mutagenesis. Genes- Traits To examine the relationship between genes and traits, researchers can alter gene sequences via site-directed mutagenesis. The altered gene is intro- duced into a living cell or organism to examine how the mutation affects the organ- ism's traits. For example, a researcher could produce a nonsense mutation in the middle of the lacY gene in the lac operon. If this site-directed mutant gene was then introduced into an E. coli bacterium that did not a have a normal copy of the lacY gene, the bacterium would be unable to use lactose. These results indicate that a functional lacY gene is necessary for bacteria to have the trait of lactose utilization.
- Oligonucleotide primer with a mismatch Gene in -Mismatch a vector (template DNA) Vector Add DNTPS, DNA polymerase, and DNA ligase. -Mismatch The DNA is introduced into a living cell, where the mismatch is repaired. GG.C CCG CTS or A site-directed mutant is made. The DNA is repaired back to the original sequence. FIGURE 21.12 The method of site-directed mutagenesis. Genes- Traits To examine the relationship between genes and traits, researchers can alter gene sequences via site-directed mutagenesis. The altered gene is intro- duced into a living cell or organism to examine how the mutation affects the organ- ism's traits. For example, a researcher could produce a nonsense mutation in the middle of the lacY gene in the lac operon. If this site-directed mutant gene was then introduced into an E. coli bacterium that did not a have a normal copy of the lacY gene, the bacterium would be unable to use lactose. These results indicate that a functional lacY gene is necessary for bacteria to have the trait of lactose utilization.
Biology: The Unity and Diversity of Life (MindTap Course List)
14th Edition
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter20: Viruses, Bacteria, And Archaea
Section: Chapter Questions
Problem 6SQ
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Describe three possible uses of site-directed mutagenesis.
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