All are correct about DNA gyrase in E. coli EXCEPT:   It works to remove positive supercoiling introduced by the DnaB protein (helicase).   It is a topoisomerase that hydrolyzes ATP during its reaction mechanism.   Its mechanism involves the breaking of a single phosphoester bond in one strand of dsDNA.   It works to relieve supercoiling in DNA to overcome the torsion stress imposed upon unwinding.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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All are correct about DNA gyrase in E. coli EXCEPT:

 
  1. It works to remove positive supercoiling introduced by the DnaB protein (helicase).

     
  2. It is a topoisomerase that hydrolyzes ATP during its reaction mechanism.

     
  3. Its mechanism involves the breaking of a single phosphoester bond in one strand of dsDNA.

     
  4. It works to relieve supercoiling in DNA to overcome the torsion stress imposed upon unwinding.

     
     
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