12) Draw a yeast knockout cassette. Label all required sequence features.  a) Draw the target chromosome if recombination only occurs at one region of homology.  b) Draw the target chromosome if recombination occurs using both regions of homology.

Q: Two homologous chromosomes in ES cells are depicted below with the portion of your gene of interest…

A: CRISPR/Cas9 creates specific double-stranded breaks at the target locus that trigger DNA repair…

Q: During X inactivation, all but one gene get silenced on one X chromosome. Why does one gene escape…

A: X inactivation This process is also called lyonization and through this process, one of the copies…

Q: 1. a)What would happen if the aminoacyl tRNA synthetase responsible for charging alanine tRNAs also…

A: The polymerization of amino acids according to the codons on mRNA to form proteins is known as…

Q: What makes up a yeast artificial chromosome (YAC)?

A: Artificial chromosomes are cloning vectors, which contain the DNA sequence elements that are…

Q: Draw a figure for the mode of transposition not shown in Figure 15-8, retrotransposition.

A: Retrotransposition occurs by LTR and Non-LTR retrotransposons.

Q: (Q134) What is the function of topoisomerase? A) To facilitate unwinding by introducing positive…

A: The process by which a DNA molecule synthesizers it's own identical copies is known as DNA…

Q: It has been shown that infectious agents such as viruses often exert a dramatic effect on their host…

A: Viruses are the obligate parasites that take over the machinery of their host cells.

Q: In yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose…

A: Telomeres play a very important role in preserving our genome information.

Q: Identical (monozygotic) twins have identical genomes. In one pair of identical twins, they inherit a…

A: Types of twins. 1. Monozygotic (identical) Twins: These twins are formed when a single fertilized…

Q: In recombinant chromosomes, where is gene conversion likely totake place: near the breakpoint or far…

A: Gene conversion is a process in which a homologous sequence is replaced by the one DNA sequence in a…

Q: Define about homologous recombination repair ?

A: The most severe form of DNA damage in eukaryotes are the double- strand breaks or broken…

Q: Why does transposition always produce direct repeats in the chromosomalDNA?

A: Transposable elements are something ubiquitious present in prokaryotes and eukaryotes and they are…

Q: Eukaryotic genomes have many copies of genes H1, H2A, H2B, and H3, but some copies have accumulated…

A: This question is based on mutations and genome.

Q: Which of the following statements about linkage disequilibrium is false?    a.) A new mutation first…

A: Linkage disequilibrium (LD) is defined as the non-random association of alleles at different loci.…

Q: You receive four strains of yeast in the mail, and the accompanying instructions state that each…

A: The expression of a gene in a eukaryote depends on various factors and is regulated by different…

Q: What is the difference between an Hfr chromosome using normal excision and aberrant excision?

A: High frequency recombination (Hfr) cells are the bacteria having a conjugative plasmid (Fertility…

Q: -PBR322 DNA (4.32 kb) was cleaved with HindIII nuclease and lig- ated to a HindlII digest of human…

A: Restriction Endonucleases cleave DNA to smaller fragments. These DNA fragments are cloned into new…

Q: An Hfr strain that is hisE + and pheA + was conjugated to a strain that is hisE − and pheA −. The…

A: Conjugation experiments are done to map different genes in bacteria. The mapped distances determined…

Q: Is homologous recombination an example of mutation? Explain.

A: Mutations are the changes in the DNA or chromosome. From changes in one base to the small fragment…

Q: What is a recombination unit in the context of the present analysis? How does it differ from the…

A: Genetics is a part of science worried about the investigation of genes, genetic variation, and…

Q: A plant geneticist was examining the inversion mutation XI 501 in barley where a segment of DNA is…

A: Any alteration occurring in the DNA sequence of an organism is known as mutation.

Q: The yeast genome has class 1 elements (Ty1, Ty2, and so forth) but no class 2 elements. What is a…

A: Yeast has a very compact genome having 70 % of its DNA as exons, therefore it contains insufficient…

Q: Discuss how ultra violet light works as a mutagen. This could include: What is UV light and the…

A: Electromagnetic radiation is an energy form that possesses both magnetic and electrical components.…

Q: The DNA fragments show a portion of two nonsister chromatids before crossover. Suppose strand…

A: Chromosomes are long thread-like structures made up of repeating DNA sequences. Any change in the…

Q: Since COVID19 RNA is able to replicate exponentially in human cells without being detected…

A: Covid is a disease caused by corona virus.  This is a epidemiological disease.

Q: Polymerase Chain Reaction (PCR) was invented by Kary Mullis in 1983. This techniqu had indeed…

A: PCR is used to amplify a specific DNA sample.

Q: Define about homologous recombination repair ?

A: Homology means similarity due to shared ancestors between pair of structures or gene in different…

Q: . Below are the DNA sequences that encode the first eight amino acids for five alleles of the Adh…

A: DNA (deoxyribonucleic acid) is the genetic material present in most organisms. The segment of DNA…

Q: What is somatic hybridisation? Describe the various steps in producing somatic hybrids from…

A: The term somatic refers to the type of cells present in the body of an organism. Cell is the…

Q: 20. Which of the following process occurs in regions where no large-scale sequence similarity is…

A: Non-homologous recombination (NHR) is a mechanism of DNA recombination that occurs in regions where…

Q: Which of the following statements regarding anabolism is TRUE? Hydrolysis reactions are generally…

A: No, it is not true that hydrolysis reactions are generally considered anabolic instead they are…

Q: Please consider the figure below, parts A and B. A В 部 蹈器 Gene B Gene C Gene B Normal Gene A…

A: According to the question, two diagrams are given and few questions are asked. We have to solve the…

Q: The figure below shows the life cycle of the fungus Neurospora. The adult stage of the Neurospora is…

A: The answer is given below.

Q: 5.) You have identified a mutant with the following phenotype:  a) Explain what has most likely…

A: Mutation is the alteration in all of part of a DNA of an organism causing the increased or decreased…

Q: 8. A mutant that has a nutritional requirement for growth is an example of a(n) A) heterotroph B)…

A: Mutation in biology is a change in the extrachromosomal genetic material, the virus, or the…

Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…

A: The end areas of chromosomes are called telomeres , are repetitive sequence that protect gene from…

Q: A reciprocal translocation occurs in an individual between chromosomes 4 and 18, and this…

A: Introduction :- Translocation is defined as the process , When any part of one chromosome detached…

Q: Knock out mice that are mutant for a gene product X die. Variants of the X gene that were…

A: The physical crossing over during meiosis of parental heterozygous homologous chromosomes or genes…

Q: was reduced in the knock-out mutant cells. The N-terminal region of Protein X is 100% conserved…

A: As per our policy we are answering the first three sub parts ( a, b , c ). Of the Question For the…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.