12) Draw a yeast knockout cassette. Label all required sequence features.  a) Draw the target chromosome if recombination only occurs at one region of homology.  b) Draw the target chromosome if recombination occurs using both regions of homology.

Parts a-e

An interrupted mating study is carried out on Hfr strains 1, 2, and 3. After conjugation is established, a small sample of the mixture is collected every minute for 20 minutes to determine the distance between the genes on the chromosome. Times of entry for each of the three Hfr strains studied in the interrupted mating are provided in parenthesis. The total duration of conjugation (in minutes) is given for each transferred gene. Hfr Strain Genes Transferred Hfr 1 oriT(0)  met(2)   ala(8)  lac(13)  gal(17) Hfr 2 oriT(0)  met(2) leu(7)  thr(10)  azi(17) Hfr 3 oriT (0)  gal(3)  pro(8)  trp(14)  azi(19) Hfr 4 oriT leu met ala lac Hfr 5 oriT trp azi thr leu a. Use the data to construct a circular map of the bacterium.

8) Transposons have two methods of transposition. Briefly describe each one and indicate whether genome is the same size or has increased in size after a transposition event.