Enzyme kinteics of amylase using l2 in KI was used to determine starch conc at t = 0 min andt = 1 min. Tubes were set absorbance measured after 1 minute of incubation. The reactionwas halted with HCl and 5 mL of I, in KI indicator was added. 1 ml of each was used assample in the spectrophotometer.Using this table,Concentration ofVolume ofVolume ofVolume of l2Test tube stock starch,in KI solution, Absorbancestarchsolution, mL water, mLdistilledmg/mLmL11050.00092100.44.60.33063100.84.20.5891410|1.23.80.84095101.63.41.08856.102.03.01.2572What is the standard curve for the conc. of the starch?

Here, from the given data the information needed to obtain the standard curve of the concentration…

Answered: Enzyme kinteics of amylase using l2 in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

A paste of starch was tested using Benedict’s test, the Iodine Test, and Fehling’s Test. Before hydrolysis, Benedict’s test and Fehling's test results were both negative while Iodine’s test is positive. After adding 0.5 mL of conc. HCl to the paste, another 5 drops of NaOH were added and was tested using Fehling’s, Benedict’s Test, and Iodine Tests. What would be the theoretical result of the three different tests? Explain the chemical reaction that happened and the principle/mechanism behind the reactions.
How do I calculate the final concentration? I just need one solution to walk through the steps. Prepare eight standards of glucose with your 1 mM glucose solution, each with a final volume of 10 mL. Complete the table on the next page, indicating what your final concentration of glucose will be for each standard, in mM. Volume of Potassium Ferrocyanide Solution Volume of Enzyme Solution Volume of 1 mM glucose solution Final volume of solution Final Concentration of glucose in final solution (mM) 2.50 mL 1.00 mL 0.00 mL 10.00 mL 2.50 mL 1.00 mL 0.50 mL 10.00 mL 2.50 mL 1.00 mL 1.00 mL 10.00 mL 2.50 mL 1.00 mL 2.00 mL 10.00 mL 2.50 mL 1.00 mL 3.00 mL 10.00 mL 2.50 mL 1.00 mL 4.00 mL 10.00 mL 2.50 mL 1.00 mL 5.00 mL 10.00 mL 2.50 mL 1.00 mL 6.00 mL 10.00 mL
I need help with my homework this question is very confusing How long would it take for a solution made by diluting 2.5 ml of #1 with 7.5 ml of H2O to react with 10 ml of #2?
The before and after fluids were observed to detect the movement of starch across the dialyzing membrane. The tables below shows the test results using chemical indicators like iodine test. BEFORE Fluid in the beaker yellow (absence of starch) Fluid in the bag black (presence of starch) AFTER Fluid in the beaker yellow (absence of starch) Fluid in the bag black (presnece of starch) Was there a movement of starch across the dialyzing membrane? Why or why not?
Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
The absorbance values for different BSA standards. How can the protein concentration be calculated? Which absorbance value should be used?
compare the graph below, compare the absorbance and wavelenght
Chlorophyll is the green pigment in the leaves of the plant, carotene imparts orange coloration while anthocyanin is known as the purple pigment. In one of their laboratory, a group was tasked to confirm the presence of these pigments on a certain leaf. After crushing several leaves, they soak it in isopropyl paper strip was dried and the group got the following measurements from the submerged tip of the paper strip: solvent font - 9.9cm, purple ink -8.6cm, yellowish orange ink - 6.4cm and green ink - 4.2 retention factor of each pigment. (d) Rank each pigment according to their solubility in the given solvent.
Sucrose + H2O(negative control): Colorless to brown solution: Blue solution Conclusion for Iodine test A. amylose present B. amylose not present Conclusion for Benedict's test A. reducing sugars not present B. reducing sugars present Sucrose + HCl(potential hydrolysis): unchanged compared to sucrose+water: Conclusion for Iodine test A. more amylose present B. LESS amylose present C. NO change Conclusion for Benedict's test a. more reducing sugar present b. less reducing sugar present c. no change Did hydrolysis of sucrose occur in the presence of acid? A. Yes B. no
A certain amount of white solid was dissolved in enough water and gives a colorless solution. A portion of the solution was added with 15% solution of alpha-naphthol solution and a few drops of conc. H2SO4 forming a violet-colored ring. Another portion was added with drops of Seliwanoff’s reagent and showed no change after being warmed in a bath. Another portion was added with equal amounts of Fehling’s A and B reagent giving a red precipitate in a blue solution after warming in a bath. a) Cite an experimental result confirming that the solid is a carbohydrate b) Is the solid starch, fructose or glucose? Support your answer.
Phenol Red Test Question: Assuming you tested an organism using Phenol Red glucose and Phenol Red Base broths, which of the combinations of results in the following table would be reliable. Interpret each of the combinations and explain how the results might be obtained
Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS