Biochemistry: The Molecular Basis of Life
Biochemistry: The Molecular Basis of Life
6th Edition
ISBN: 9780190209896
Author: Trudy McKee, James R. McKee
Publisher: Oxford University Press
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Chapter 3, Problem 7RQ
Summary Introduction

Interpretation:

For the solution of pH 8.3, the hydrogen ion (H+) concentration is to be calculated.

Concept introduction:

The pH scale is based on the concentration of hydrogen ions. It is a logarithmic scale. From the given value of pH, the concentration of hydrogen ions can be determined. The concentration of H+ ion can be calculated as follows:

pH=log[H+]pH=log[H+]

Taking 10 raised to pH and log[H+] on both the sides:

10pH=10log[H+]10pH=[H+]

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Question 1: Why do we need to add concentrated H2SO4 (sulfuric acid) or HCl (hydrochloric acid) during Molisch's Test? Question 2: Why do trioses and tetroses give negative result for the Molisch's Test? Explain with the help of necessary chemical structures or minimal reaction blocks. Question 3: Why do some organic acids like citric acids, lactic acid, etc. give positive result for Molisch's Test? Question 4: Is Molisch's Test a specific test for detecting Carbohydrates? Discuss in brief with other possible test for the detection of carbohydrates. Question 5: Suppose, you are provided with samples of commercially available sugar-free carbonated soft drinks. How would you perform the Molisch's Test to verify?

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Biochemistry: The Molecular Basis of Life

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