(a)
To explain: The effect of F2, 6BP on enzyme activity.
Introduction: Phosphofructokinase-1 is a regulatory enzyme which is involved in the process of glycolysis. It consists of several activators and inhibitors. Phosphofructokinase-1 is primarily involved in the conversion of fructose-6-phosphate and ATP into FBP.
(b)
To explain: The effect of ATP concentration on data in Figure 9-15b.
Introduction: Phosphofructokinase-1 is a regulatory enzyme which is involved in the process of glycolysis. It consists of several activators and inhibitors. Phosphofructokinase-1 is primarily involved in the conversion of fructose-6-phosphate and ATP into FBP.
(c)
To explain: The assumptions about ATP concentrations in Figure 9-15a and also explain about the concentration of fructose-2, 6-biphosphate in Figure 9-15b
Introduction: Phosphofructokinase-1 is a regulatory enzyme which is involved in the process of glycolysis. It consists of several activators and inhibitors. Phosphofructokinase-1 is primarily involved in the conversion of fructose-6-phosphate and ATP into FBP.
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Becker's World of the Cell (9th Edition)
- Palmitoleic acid, 16:1Δ⁹ hexadecaenoic acid, (16 carbon FA with one double bond )is an important fatty acid component of TAGs and cell membranes. Briefly explain the process of beta oxidation of this fatty acid and the number (only) of FADH, NADH and acetyl CoA outcome. What is the total ATP (only number) generated from this fatty acid after beta oxidation.arrow_forwardInducers and Inhibitors of AEP. Short peptides such as legumain stabilization and activity modulation (LSAM) domain and αvβ3 integrin could enhance the activity of AEP. LSAM domain known as the prodomain of AEP blocks substrate binding before activation. This prodomain has a helical structure and two independent peptides. One is an activation peptide (AP, K287 to N323), and the other is a LSAM domain. LSAM domain remains even after AP is cleaved and released from protease at neutral pH via electrostatic interaction. AEP without LSAM domain has a lower melting temperature than AEP with LSAM domain [77, 117]. Another short peptide, αvβ3 integrin, can directly interact with AEP, and after forming a complex, the optimal pH for AEP activity is increased from 5.5 to 6.0. It indicates that αvβ3 binding could induce conformational stabilization of AEP accompanied by deprotonated C189. αvβ3 does not directly interact with the AEP active site; however, AEP docks to the αvβ3 RGD-binding site…arrow_forwardThe Cori Cycle. Before vigorous exercise (at rest) the level of blood lactate is at its normal level (about 25 mM). During a 400 m sprint, the value rises sharply in less than a few minutes to about 200 mM and then declines slowly to around 40 mM over 60 mins after the sprint. a. Discuss (or illustrate) the pathway and reactions that cause the rapid increase in lactate concentration during the sprint. b. What causes the slow decline in lactate concentration after the sprint? Why does the decrease occur more slowly than the rapid increase? c. What enzymatic reaction is responsible for maintaining the lactate concentration above zero at recovery after the sprint?arrow_forward
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning