Concept explainers
Using the DNA sequence 3′ TAC CAG ATA CAC TCC CCT GCG ACT 5′ illustrate and explain the following mutations:
- a. a deletion
- b. an insertion
- c. a substitution
- d. a nonsense mutation
- e. a frameshift mutation
a.
To explain: A deletion mutation by using DNA sequence.
Introduction:
Mutation in an organism is defined as a sudden alteration of “nucleotide sequences”. The mutations can be harmful, beneficial or neutral depending on the alteration in the genome and the effect exerted by the environment.
Explanation of Solution
Deletion mutation involves the removal of single base results in a frameshift mutation that changes the reading frame. Deletion mutation in given DNA sequence is:
b.
To explain:
An insertion mutation by using DNA sequence
Introduction:
The mutation causes an alteration in the nucleotide sequence of DNA. It is caused by natural as well as artificial factors. Mutagens are the agents that cause mutation in DNA. Mutation is a permanent change in the DNA sequence.
Explanation of Solution
The insertion mutation involves the addition of one or more nucleotide sequences in a gene that alters the sequence and results in changes the protein function. An insertion mutation in a given DNA sequence
c.
To explain:
A substitution mutation by using the given DNA sequence
Introduction:
Mutation is defined as a change in the sequence of genetic material either due to the mistake in replication or due to the changes in the environment. DNA enzymes have a property of proofreading which prevents any mistake in a nucleotide sequence. But sometimes some mistakes during DNA replication are undetectable. These alterations in the nucleotide sequences are known as mutation.
Explanation of Solution
It involves the replacement of one base pair of DNA with another. Such type of mutation is silent as the mutated gene encodes for the same protein as the original gene. It is also called missense mutation. Due to substitution, the shape of amino acids is changed and its function also changes. For example sickle cell anemia. A substitution mutation in a given DNA sequence
d.
To explain:
A non-sense mutation in a given DNA sequence
Introduction:
Mutation is a sudden unpredictable change in the DNA sequence. It causes an alteration in the sequence of DNA. It is caused by natural factors or by exposure to certain artificial mutagens.
Explanation of Solution
The nonsense mutation occurs when base triplet that codes for a specific amino acid changes into stop codon and terminates the process of translation. Non-sense mutation in a given DNA sequence is
e.
To explain:
A frameshift mutation in a given DNA sequence
Introduction:
Mutation is defined as the change in the nucleotide sequence of the gene which is results in a change in the coding of a different protein. Each codon codes for specific amino acid. It occurs due to physical and chemical mutagens.
Explanation of Solution
Insertion and deletion is a type of gene mutation which involves either addition or removal of one or more nucleotides bases from a sequence of DNA. Insertion and deletion mutation together lead to cause frameshift mutation. Insertion mutation occurs when nucleotides bases are inserted into the sequence of DNA whereas deletion mutation occurs when one or more nucleotide bases are deleted from DNA sequence. A frameshift mutation in DNA sequence
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Chapter 9 Solutions
Microbiology: A Systems Approach
- If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense mutation cause a protein to be shorter, longer, or the same length as the normal 700 amino acid proteins? What would be the effect of a nonsense mutation? A sense mutation?arrow_forwardA researcher was mutating prokaryotic cells by inserting segments of DNA. In this way, she made the following mutation:Original TTGACAT 15 to 17 bp TATAATMutant TATAAT 15 to 17 bp TTGACATa. What does this sequence represent?b. What do you predict will be the effect of such a mutation? Explain.arrow_forwardOriginal DNA template: 3'-ACGGTCAATTTGCTG-5 a) Transcribe the sequence. b) Translate the sequence. c) What type of mutation is present in the strand 3 '- ACGGTCAATATTGCTG - 5 d) Provide the entire mutated sequence of amino acids. e) Explain the effect that this mutation will have.arrow_forward
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