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QUANTITATIVE Turnover Number. Carbonic anhydrase catalyzes the reversible hydration of carbon dioxide to form bicarbonate ion:
As we will discover in Chapter 8, this reaction is important in the transport of carbon dioxide from body tissues to the lungs by red blood cells. Carbonic anhydrase has a molecular weight of 30,000 and a turnover number (kcat value) of 1 × 106 sec−1. Assume that you are given 1 mL of a solution containing 2.0 μg of pure carbonic anhydrase.
- (a) At what rate (in millimoles of CO2 consumed per second) will you expect this reaction to proceed under optimal conditions?
- (b) Assuming standard temperature and pressure, how much CO2 is that in mL per second?
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Becker's World of the Cell (9th Edition)
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning