Concept explainers
Temperature and pH Effects. Figure 6-4 illustrates enzyme activities as functions of temperature and pH. In general, the activity of a specific enzyme is highest at the temperature and pH that are characteristic of the environment in which the enzyme normally functions.
- (a) Explain the shapes of the curves in Figure 6-4 in terms of the major chemical or physical factors that affect enzyme activity.
- (b) For each enzyme in Figure 6-4, suggest the adaptive advantage of having the enzyme activity profile shown in the figure.
- (c) Some enzymes have a very flat pH profile—that is, they have essentially the same activity over a broad pH range. How might you explain this observation?
(a) Temperature dependence. The reaction rate for both a typical human enzyme (black) and a typical enzyme from a thermophilic bacterium (green) varies with temperature. It is highest at the optimal temperature, which is about 37°C (body temperature) for the human enzyme and about 75°C (the temperature of a typical hot spring) for the bacterial enzyme. Above the optimal temperature, the enzyme is rapidly inactivated by denaturation.
(b) pH dependence. The reaction rate of an enzyme is highest at its optimal pH, which is about 2.0 for pepsin (stomach pH) and near 8.0 for trypsin (intestinal pH). At the pH optimum for an enzyme, ionizable groups on both the enzyme and the substrate molecules are in the most favorable form for reactivity.
Figure 6-4 The Effect of Temperature and pH on the Reaction Rate of Enzyme-Catalyzed Reactions. Every enzyme has an optimum temperature and pH that usually reflect the environment where that enzyme is found in nature.
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Becker's World of the Cell (9th Edition)
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