The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter. Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

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Answer 1

Question

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

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ENZYME KINETICS ANALYSIS of 6 Xanthine oxidase (XO) is the enzyme that catalyzes the synthesis of uric acid, which in excess causes gouty arthritis. The inhibition of this enzyme is therefore critical in its treatment. A student researcher is investigating the inhibitory effects of kaempferol (Kmp) and chlorogenic acid (Cha) on XO which uses xanthine (Xan) as substrate. Table 1 below shows the enzyme kinetic data. Construct the Lineweaver-Burk plot complete with the linear regression analvsis. Fill in the needed information on Table 2 and paste a copy of your Lineweaver-Burk plot. submit the picture of your output in PNG or JPG format. Table 1. Enzyme Kinetic Data Velocity, mM/s [S], mM Хan Kmp Cha 0.492 0.0678 0.0351 0.0615 0.211 0.0531 0.0261 0.0451 0.087 0.0298 0.0157 0.0211 0.048 0.0195 0.0091 0.0142 0.029 0.0127 0.0067 0.0081 Table 2. Enzyme Kinetic Parameters Xanthine Kaempferol Chlorogenic acid Parameters Vmax Км Type of Inhibition Mode of Binding NA NA Lineweaver-Burk Plot

Based on the Lineweaver-Burke plot attached. Kinetic data were generated in the (1) absence of any inhibitor, (2) presence of 15 µM of a reversible inhibitor, or (3) presence of 20 µM of a second (distinct) reversible inhibitor. Purified enzyme concentration was 5 µM. The y-intercept of Lines (A) and (B) is 0.9 sec/uM; the y-intercept of Line (C) is 0.3 sec/uM. The slope of Line (A) is 1.8 sec; the slope of Lines (B) and (C) is 0.6 sec. Calculate the Km of the reaction represented by Line (B).

Based on the Lineweaver-Burke plot attached. Kinetic data were generated in the (1) absence of any inhibitor, (2) presence of 15 µM of a reversible inhibitor, or (3) presence of 20 µM of a second (distinct) reversible inhibitor. Purified enzyme concentration was 5 µM. The y-intercept of Lines (A) and (B) is 0.9 sec/uM; the y-intercept of Line (C) is 0.3 sec/uM. The slope of Line (A) is 1.8 sec; the slope of Lines (B) and (C) is 0.6 sec. Calculate the catalytic efficiency of the reaction represented by Line (A).

Answer 2

Question

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

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Answer 3

Question

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Expert Solution & Answer

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Answer 4

Question

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Expert Solution & Answer

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Answer 5

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 6

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 7

Chapter 6, Problem 6.13PS

(a)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 9 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 8

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 9

(b)

Summary Introduction

To explain: The effect of change in concentration on overall rate and also compare the effect on first tripling of the concentration.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 10

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 11

(c)

Summary Introduction

To determine: The overall rate of shelling for the ten monkeys if the peanut concentration is increased to 90 peanuts per square meter.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 12

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 13

(d)

Summary Introduction

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 14

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 15

(e)

Summary Introduction

To draw: A graph with v on y-axis and [S] on x-axis and also predict V_max and K_m.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 16

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 17

(f)

Summary Introduction

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

Answer 18

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Answer 19

Expert Solution & Answer

Answer 20

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Answer 21

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To explain: The effect of change in concentration to tenfold on the overall rate. Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

To draw: A graph with v on y-axis and [S] on x-axis and also predict Vmax and Km. Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

To determine: The value of Km for shelling reaction. Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

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Chapter 6 Solutions

To explain: The effect of change in concentration to tenfold on the overall rate.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.

To determine: The value of K_m for shelling reaction.

Introduction: The monkeys shelling peanuts is an example to understand the enzyme –substrate reaction where, monkeys are referred as the enzymes and peanut concentration is referred as the substrate concentration.