Biochemistry: Concepts and Connections (2nd Edition)
2nd Edition
ISBN: 9780134641621
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 6, Problem 15P
It has been postulated that the normal (noninfectious) form of prion differs from the infectious form only in secondary/tertiary structure.
a. How might you show that changes in secondary structure occur?
b. If this model is correct, what are the implications for structural prediction schemes?
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It has been postulated that the normal (noninfectious) form of prion differsfrom the infectious form only in secondary/tertiary structure.(a) How might you show that changes in secondary structure occur?(b) If this model is correct, what are the implications for structural prediction schemes?
Various antimicrobial drugs to treat microbial infection have diverse mechanism of action. Consider the following antimicrobial drugs:
A. Seconeolitsine, known as DNA topoisomerase I inhibitor in bacteria.
(i) Explain briefly how inhibiting DNA topoisomerase I is a good mechanism of action for an antibiotic, include possible molecular machineries being targeted.
(ii) What would be an appropriate response if seconeolitsine works well by stating the state of supercoiling in bacteria.
(iii) To prove your answer (ii), you test the condition of bacterial DNA by running gel electrophoresis, one has been treated with seconeolitsine (+ sample) and the other one is not (- sample). Explain the position of each + sample and – sample band on the gel in reference to the point of origin (where you load your samples) or how far each DNA sample travel across agarose gel.
(iv) Explain why you would expect answer (iii) for each + sample and – sample.
B.…
Among the following statements regarding the use of viral vectors, which is (are) true: (can be more then one answer)
A.Viral vector transfection has the defect of rapidly killing cells, given the high toxic potential of viruses.
B.Generally, the effectiveness of a viral infection transfection is much higher than an electroporation or cationic liposome transfection.
C.Even before infecting a cell, a virus can begin to express the indicators, accelerating the process of expression of the indicator in the culture medium.
D.Adeno-associated viruses are little used in biophotonics, as they require simultaneous adenovirus infection.
Chapter 6 Solutions
Biochemistry: Concepts and Connections (2nd Edition)
Ch. 6 - Prob. 1PCh. 6 - Bovine pancreatic trypsin inhibitor (BPTI; Figure...Ch. 6 - A schematic structure of the subunit of...Ch. 6 - In the protein adenylate kinase, the C-terminal...Ch. 6 - Give two reasons to explain why a proline residue...Ch. 6 - Consider a small protein containing 101 amino acid...Ch. 6 - a. Based on a more conservative answer to Problem...Ch. 6 - The following sequence is part of a globular...Ch. 6 - a. A protein is found to be a tetramer of...Ch. 6 - Under physiological conditions, the protein...
Ch. 6 - Theoretical and experimental measurements show...Ch. 6 - The peptide hormone vasopressin is used in the...Ch. 6 - A protein gives under conditions of buffer...Ch. 6 - A protein gives a single band on SDS get...Ch. 6 - It has been postulated that the normal...Ch. 6 - Below are shown two views of the backbone...Ch. 6 - Do you expect a Pro Gly mutation in a...Ch. 6 - Rank the following in terms of predicted rates...Ch. 6 - Shown below are two cartoon views of the small...Ch. 6 - Prob. 20PCh. 6 - In most cases, mutations in the core of protein...Ch. 6 - A Leu Ala mutation at a site buried the core of...Ch. 6 - Disulfide bonds have been shown to stabilize...Ch. 6 - Cartoon renderings of the proteins Top 7 and adaH2...
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