Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 57, Problem 4Q
Summary Introduction
To review:
The problem of hypoxia in aquaculture operations and the effect of total organic carbon (TOC) on oxygen levels.
Given:
Aquaculture farming such as mussel farming can cause hypoxia or low level of oxygen, which is available in water.
Introduction:
Aquaculture farming, which is also known by the name of aquafarming, is the farming, which involves rearing of mollusks, fishes, plants, crustaceans, algae, etc. for a commercial purpose. The concern with aquaculture is the amount of waste and organic matter, which is produced by it, which can eventually cause pollution if these substances get released to into the coastal waters or other aquatic ecosystems.
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A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air.
What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air.
a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)?
b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration?
c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
The data in the table below was collected by researchers to investigate the oxygen concen-
tration of water in a lake. Data was collected from the location in the lake at the same time
every day repeatedly over a six-day period.
Day
A
B
с
D
a histogram
a ple chart
a bar graph
Day 1
aline graph
Day 2
Day 3
Day 4
Day 5
Which of the following is the most appropriate graph to display the data in the table?
Day 6
Oxygen Concentration (ppm)
1.2
21
20
1.7
1.5
Chapter 57 Solutions
Life: The Science of Biology
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