Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 5.7, Problem 2TQ
Summary Introduction
To analyze:
The number of viable cells left after 4 minutes of disinfectant exposure into a culture containing 1X106 CFUs per milliliter and the D-value of the disinfectant is 2 minutes.
Introduction:
Microbes are microscopic organisms that are visible under the microscope. Some microbes are friendly in nature while others are harmful. The growth of harmful microbes is controlled or inhibited by various methods such as sterilization, disinfection, antisepsis, and sanitation. Disinfectants are chemical agents or liquids that are used to kill or eliminate pathogenic organisms.
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Chapter 5 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 5.2 - Prob. 1TQCh. 5.2 - Prob. 2TQCh. 5.2 - Prob. 3TQCh. 5.3 - Prob. 1TQCh. 5.4 - Prob. 1TQCh. 5.5 - Prob. 1TQCh. 5.5 - Prob. 2TQCh. 5.5 - Prob. 3TQCh. 5.6 - Prob. 1TQCh. 5.7 - Prob. 1TQ
Ch. 5.7 - Prob. 2TQCh. 5.7 - Prob. 3TQCh. 5 - Prob. 1RQCh. 5 - Prob. 2RQCh. 5 - Prob. 3RQCh. 5 - Prob. 4RQCh. 5 - Prob. 5RQCh. 5 - Prob. 6RQCh. 5 - Prob. 7RQCh. 5 - Prob. 8RQCh. 5 - Prob. 9RQCh. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Prob. 12RQCh. 5 - Prob. 1TQCh. 5 - Prob. 2TQCh. 5 - Prob. 3TQCh. 5 - Prob. 4TQCh. 5 - Prob. 5TQ
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- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?arrow_forwardWhat is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 x 106 cells/mL?arrow_forwardHow would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.arrow_forward
- Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?arrow_forwardA bacterial culture has a concentration of 3.2 x 108 cells /mL. You dilute this culture as follows: 1/50, then 10-3 and finally 1/20. If you then plate 0.2 mL of the final dilution, how many CFU would you expect following incubation?arrow_forwarda. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.arrow_forward
- Serial dilution = previous dilution x dilution 11 mL [2] 3 mL Colony count: 100 0.1 mL sample [1] 9.9 mL 5 mL 0.1 ml. Cokony count Dilution: 10-1 10-3 [3] 150 Dilution factor (DF): [4] [5] [6] Compute for the CFU/mL of the sample. Show your solution and express your final answer to 2 significant figures.arrow_forwardA cell population has 10,000,000 cells and you add a disinfectant. Assume the constant D-value is 5 minutes. How many cells will be left after 10 minutes?arrow_forwardYou do a serial dilution on an actively growing bacterial culture broth. Doing a plate count of your final serial dilution gives you 30 CFU/mL. What is your original concentration of bacterial culture broth if you completed two 1:5 serial dilutions?arrow_forward
- What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?arrow_forwardA sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.arrow_forwardAssume an inoculum with a cell density of 108 cells per mL. The entire generation time takes 30 minutes. How many hours would it take to grow a culture to 108/mL if you started with a 10–2 dilution? helpful formula: g (generation time) = 0.301 (time)/ log x – log xoarrow_forward
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