Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 5, Problem 4TQ
Summary Introduction
To review:
The efficiency of phage therapy as a solution to antibiotic resistance.
Introduction:
Antibiotics are the chemical compounds which are used to destroy, slow down, or inhibit bacterial growth. Antibiotic resistance is the ability of bacteria to survive even in the presence of antibiotics. This resistance occurs when the bacteria become modified to overcome the antibiotic effects.
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Chapter 5 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 5.2 - Prob. 1TQCh. 5.2 - Prob. 2TQCh. 5.2 - Prob. 3TQCh. 5.3 - Prob. 1TQCh. 5.4 - Prob. 1TQCh. 5.5 - Prob. 1TQCh. 5.5 - Prob. 2TQCh. 5.5 - Prob. 3TQCh. 5.6 - Prob. 1TQCh. 5.7 - Prob. 1TQ
Ch. 5.7 - Prob. 2TQCh. 5.7 - Prob. 3TQCh. 5 - Prob. 1RQCh. 5 - Prob. 2RQCh. 5 - Prob. 3RQCh. 5 - Prob. 4RQCh. 5 - Prob. 5RQCh. 5 - Prob. 6RQCh. 5 - Prob. 7RQCh. 5 - Prob. 8RQCh. 5 - Prob. 9RQCh. 5 - Prob. 10RQCh. 5 - Prob. 11RQCh. 5 - Prob. 12RQCh. 5 - Prob. 1TQCh. 5 - Prob. 2TQCh. 5 - Prob. 3TQCh. 5 - Prob. 4TQCh. 5 - Prob. 5TQ
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- One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forwardWhich of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar platearrow_forwardClassify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.arrow_forward
- phage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnductionarrow_forwardAntibotic/drug resistance in bacteria results from mutations induced by the anitbiotic which enhance fitness of the bacteria ensuring their survival. a) True b) Falsearrow_forwardIdentify examples of cell-wall antibiotics that are not beta-lactam drugs.arrow_forward
- Provide a brief explanation on the idea of genetic engineering in formulating an antiviral from a single cell organismsarrow_forwardLambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).arrow_forwardYou are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forward
- The acquired antibiotic resistance genes encode what types of proteins that can inhibit the function of the antibiotic? list twoarrow_forwardbriefly describe four explanations why E. coli phages can only infect E. coli cells, but not other bacteria or human cells.arrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forward
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