Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 35, Problem 8P
Interpretation Introduction
(a)
Interpretation:
The smallest unit of the antibody having the specificity and binding affinity of the whole protein should be determined.
Concept introduction:
The antibodies are the immunoglobulins (Ig) made up of two types of chains that is a light chain and heavy chain. The immunoglobulin is a class of proteins found in the plasma of the animals. The typical structure of the antibody is given below:
Interpretation Introduction
(b)
Interpretation:
A compact single chain that is likely to bind with antigen should be designed.
Concept introduction:
The antibodies are the immunoglobulins (Ig) made up of two types of chains that is a light chain and heavy chain. The immunoglobulin is a class of proteins found inthe plasma of the animals. The antigens are the substance that can elicit an immune response by binding to the antibody.
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Biomedical Engineering:
An equilibrium dialysis experiment was performed to characterize the binding affinity of a mouse IgG or human IgG antibody for an antigen, GAD65. (The association constant for the monoclonal mouse IgG is 4.75 x 10^8 M-1 and for a human IgG is 1.3 x 10^10 M-1)
a- calculate the dissociation constant for each antibody and explain which antibody has a higher binding affinity for GAD65?
b-calculate the fraction of free Ab sites for each antibody for a For a GAD65 concentration of 0.7 nM
Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels.
1. Immunoglobulin G in a non-reducing gel (no β-mercaptoethanol added in the sample solution) – the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da.
Predict the number of bands and apparent mol. wt. of the following proteins on SDS gels.
1. Immunoglobulin G in a reducing gel (β-mercaptoethanol added in the sample solution) – the light chains have a molecular weight of 25,000 Da and the heavy chains 50,000 Da.
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