Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 35, Problem 3P
Interpretation Introduction
Interpretation:
The difference between affinity and avidity and for which class avidity is important should be determined.
Concept introduction:
The antibodies are the immunoglobulins (Ig) made up of two types of chains that is a light chain and heavy chain. The immunoglobulin is a class of proteins found in the plasma of the animals. In humans, there are five classes of immunoglobulin, IgA, IgD, IgE, IgG, and IgM.
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It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
Need help.
Please explain "droplet based immunoassay(immune assay)".
Including "single droplet generates one phase of system".
Can Ab. Act as antigens? Explain why.
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- Neutralizing antibodies are effective at preventing infection or toxicity mediated by pathogens or their toxic products. In fact, nearly all vaccines currently in use function by eliciting neutralizing antibodies. One example is the tetanus vaccine, in which neutralizing antibodies are generated against an inactivated form of the tetanus toxin (the tetanus toxoid). The most important feature of a neutralizing antibody is having high affinity for the antigen. being efficient at activating the complement cascade. having a high degree of multivalency, such as being a pentamer or hexamer of immunoglobulin monomers. being present at a high concentration in the circulation. 0 0 0 0arrow_forwardPlease help me I really a comprehensive explanation please help. Talk about covid and why it’s a problem and what it is ?? Discuss our immune system and the type of immune systems people can have as well as what antibodies are ?? What is about vaccination and what type of vaccinations we use here in NZ for covid ??arrow_forwardIrradiation of mice with a dose of 600 rad of total body irradiation eliminates 95% of total lymphocytes from spleen and lymph nodes and also eliminates all antigen-specific memory B cells. Nonetheless, when Influenza A-infected mice are subjected to this irradiation at 60-days post-infection, and then reconstituted with bone marrow cells from a naive mouse (this replenishes all of the lymphocyte populations), the levels of circulating anti-Influenza A IgG antibodies show nearly no decline when mice are monitored for the following year. What is the explanation for this finding?arrow_forward
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