Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 35, Problem 9P
Interpretation Introduction

(a)

Interpretation:

The mechanism of B cell activation should be determined. 

Concept introduction:

The B cells are the immune cells, which are responsible for the production of the immunoglobulins or the antibodies. The antigens are the particles, which elicit an immune response on binding with antibodies.

Interpretation Introduction

(b)

Interpretation:

The use of antibodies to activate b cells should be determined. 

Concept introduction:

The B cells are the immune cells, which are responsible for the production of the immunoglobulins or the antibodies. The antigens are the particles, which elicit an immune response on binding with antibodies.

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MCQ. It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate? a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
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