Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 35, Problem 12P
Interpretation Introduction
Interpretation:
The important of HLA matching for organ transplantation should be identified.
Concept introduction:
The organ transplantation or the tissue transplantation is the process of replacing the damaged body part with the body part of another person or organism. As in transplant, the cells from the outside of the body is introduced, there are chances of immune rejection and destruction of the transplanted part by the host immune system.
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It is possible to incubate your tissue with two different primary antibodies simultaneously. If this is done, it is imperative that you can track each primary antibody with a different color. For example, one secondary antibody is tagged with Alexa488 which emits as green and another secondary antibody is tagged with Alexa 555 which emits as orange. Which of the following immunohistochemistry protocols is appropriate?
a. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 secondary antibody
b. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 488 and anti-chicken Alexa 555 secondary antibodies
c. Using Rabbit anti-GFAP and Chicken anti-S100beta, followed by anti-rabbit Alexa 555 and anti-chicken Alexa 555 secondary antibodies
d. Using Rabbit anti-GFAP and Rabbit anti-S100beta, followed by anti-rabbit Alexa 488 and anti-rabbit Alexa 555 secondary antibodies
Need help.
Please explain "droplet based immunoassay(immune assay)".
Including "single droplet generates one phase of system".
In own word. What are the differences between contagious and communicable diseases? Explain with example?
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