Study Guide for Campbell Biology
11th Edition
ISBN: 9780134443775
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Jane B. Reece, Martha R. Taylor, Michael A. Pollock
Publisher: PEARSON
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Chapter 27, Problem 4TYK
Summary Introduction
Introduction: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction, transformation, and conjugation. The mechanism of conjugation involves contact of cells in which the genetic material is transferred between the two cells having different mating types.
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Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pillin protein
Genetic information can be transferred horizontally from one bacterial cell to another cell of the "same generation" (versus daughter cells), leading to genetic recombination, by all of the following EXCEPT:
A. Gene expression (transcription/translation/protein synthesis)
B. Transformation
C. Conjugation
D. Transduction
E. None of the other four answers (all are mechanisms of “horizontal transfer” genetic recombination)
For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
Chapter 27 Solutions
Study Guide for Campbell Biology
Ch. 27 - Fill in the following table with brief...Ch. 27 - Complete the following concept map that summarizes...Ch. 27 - This is a micrograph of the cyanobacterium...Ch. 27 - Prob. 4IQCh. 27 - Prob. 5IQCh. 27 - One might think of prokaryotes as primitive,...Ch. 27 - Prob. 2SYKCh. 27 - Prob. 1TYKFCh. 27 - __________ region in which the prokaryotic...Ch. 27 - __________ common staining technique that...
Ch. 27 - Prob. 4TYKFCh. 27 - Prob. 5TYKFCh. 27 - Prob. 6TYKFCh. 27 - Prob. 7TYKFCh. 27 - Prob. 8TYKFCh. 27 - ___________ use of organisms to remove...Ch. 27 - __________type of respiration that uses molecules...Ch. 27 - The Gram stain distinguishes among bacteria based...Ch. 27 - Prob. 2TYKCh. 27 - Some prokaryotes have specialized internal...Ch. 27 - Prob. 4TYKCh. 27 - Prob. 5TYKCh. 27 - Prob. 6TYKCh. 27 - Prob. 7TYKCh. 27 - Prob. 8TYKCh. 27 - Prob. 9TYKCh. 27 - Prob. 10TYKCh. 27 - Which of the following statements accurately...Ch. 27 - Prob. 12TYKCh. 27 - O157:H7, a pathogenic strain of E. coli, has over...
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- What happens when T2 phages are grown with radioactive phosphorus? a. Their DNA becomes radioactive. b. Their proteins become radioactive. c. Their DNA is found to be medium density in a centrifuge tube. d. They are no longer able to transform bacterial cells. e. They transfer their radioactivity to E. coli chromosomes during infection. ...Explain your answer.arrow_forwardConjugation involves which of the following: a. transfer of plasmids among bacteria b. sexual reproduction of Eukaryotes c. uptake of free DNA from the environment d. integration of a virus into a chromosomearrow_forwardChoose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct? a. During conjugation, two bacteria exchange genetic information directly via pili b. Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability c. Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less d. Plasmids carry just a few genes and are replicated independently from the bacterial chromosome e. Cocci that are arranged in clusters/bunches are called staphylococciarrow_forward
- During transduction via phage P1,a. any small fragment of the bacterial chromosome may betransferred to another bacterium by a phage.b. only a specific fragment of DNA may be transferred to anotherbacterium by a phage.c. any small fragment of the bacterial chromosome may betransferred during conjugation.d. only a specific fragment of DNA may be transferred duringconjugation.arrow_forwardFor 'transformation' to occur in bacterial cells, certain conditions must be satisfied. Check all the conditions that apply--Select one or more: a.low nutrient availability b.high cell density c.presence of different bacterial species/strains d.low cell density e.presence of antibioticsarrow_forwardReferring to conjugation in bacteria: Mark all cell types for which the following statement is true. Cell is used as a donor cell in conjugation. Select one or more: O a. F+ O b. F- O c. Hfr O d. F"arrow_forward
- Which of the following statements about conjugation is false? After a F- cell has conjugated with an F+ cell, both cells would be F+. Both Hfr and F+ bacteria have the ability to produce a sex pilus. In F+ × F- conjugation, none of the bacterial chromosome is transferred; only the F factor is. A Hfr strain usually transfers the entire bacterial chromosome to the recipient cell. F' × F- conjugation can produce a partially diploid bacterial cell.arrow_forwardThe likelihood of co-transformation of two bacterial genes increases: a. As their distance apart on the chromosome increases b. As their distance apart on the chromosome decreases c. As the transducing phage enter the lytic cycle d. As recombination rate decreases e. None of these is correcarrow_forwardIn Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make? A. Protein does not enter the bacterium during transduction. B. DNA does not stay in the media after transduction. C. All of the phage DNA was radiolabeled. D. Bacteria can be transduced by phage.arrow_forward
- A transducing particle injects all of the DNA that it contains into a bacterial cell. Which of the following are TRUE? A. The bacterium becomes Hfr B. The bacterium becomes F+ C. The bacterium immediately acquires new functions as the injected DNA is rapidly transcribed and translated into mRNA and proteins D. This injected DNA can integrate into the bacterium’s genome through a process called recombinationarrow_forwardA particular strain of λ (lambda) can lysogenize its E. coli host at 30°C, but not at 42°C. Could a temperature-sensitive mutation in the int (integrase) gene explain this phenotype? A. There is insufficient information to answer the question. B. No C. Yesarrow_forwardThe primary advantage of conjugation is that ita. is the first step in the formation of a biofilm.b. allows prokaryotes to inject toxins into host cells.c. shuffles genes—including some that enhance survival—among cells.d. provides a mechanism by which bacteria avoid being infected withviruses.arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license