(a)
Interpretation:
The effect of increasing the mass of stationaryphase relative to the packing masson the plate height needs to be explained.
Concept introduction:
As the number of plates increases in a GC column, the resolution would also increase. For increasing the number of plates, the height of the plates has to be decreased for maintaining a high resolution. The van Deemter equation is used for defining the effect of different parameters on the height of plate.
(b)
Interpretation:
The effect of decreasing the rate of sample injection on the plate height needs to be explained.
Concept introduction:
The resolution will increase as the number of plates increases in the GC column. For maintaining a high resolution and increasing the number of plates, the height of the plates must be decreased. For defining the effect of different parameters on the height of plate, van Deemter equation is used.
(c)
Interpretation:
The effect of increasing the injection port temperature on the plate height needs to be explained.
Concept introduction:
In GC column resolution will increases as the number of plates increases.For maintaining a high resolution and increasing the number of plates, the height of the plates has to be decreased. For defining the dependencies of various parameters on the height of plate, van Deemter equation is used.
(d)
Interpretation:
The effect of increasing the flow rate on the plate height needs to be explained.
Concept introduction:
As the number of plates increases in a GC column, the resolution would also increase. For increasing the number of plates, the height of the plates has to be decreased for maintaining a high resolution. The van Deemter equation is used for defining the effect of different parameters on the height of plate.
(e)
Interpretation:
The effect of reducing the particle sizeof the packing on the plate height needs to be explained.
Concept introduction:
The resolution will increase as the number of plates increases in the GC column. For maintaining a high resolution and increasing the number of plates, the height of the plates must be decreased. For defining the effect of different parameters on the height of plate, van Deemter equation is used.
(f)
Interpretation:
The effect of decreasing the column temperature on the plate height needs to be explained.
Concept introduction:
In GC column resolution will increases as the number of plates increases. For maintaining a high resolution and increasing the number of plates, the height of the plates has to be decreased. For defining the dependencies of various parameters on the height of plate, van Deemter equation is used.
Trending nowThis is a popular solution!
Chapter 27 Solutions
Principles of Instrumental Analysis
- 6.) An unknown polymer sample is fractionated, and the following molar fractions are obtained. Calculate Mn and Mw and the dispersity. Fill out the table to show your work. Fraction # 1 2 3 4 5 6 n₁ (mol) 0.004 0.011 0.009 0.003 0.017 0.008 M; (g/mol) 10,400 12,080 13,950 16,700 18,150 20,000 n¡Mi n;M;²arrow_forwardQ4 (a) Analyze the importance of dropping mercury electrode on polarography. (b) (i) Calculate the molarity of MgSO4 in a solution containing 1.5 g ofMgSO4 in a volume of 250 mL?(ii) Calculate the weight of MgSO4 in grams which is present in 100mL of 0.076 M MgSO4?(c) Evaluate the key differences between paper chromatography and gaschromatographyarrow_forwardAnswer each of the following by True or False. Q1) Solution resistance varies as the frequency of the applied voltage charges, then resistance is normally named as impedance.( ) Q2) Physisorption or physical adsorption is a type of adsorption in which the adsorbate adheres to the surface only through Van der Waals.( ) Q3) Michelson interferometer is considered energy dispersive spectrometer for spectral analysis.( ) Q4) lodine when mixed with starch gives deep red complex.( ) Q5) (BET) theory aims to explain the physical adsorption of gas.( ) Q6) The iodination of acetone can be easily investigated because iodine has a deep green color.( ) Q7) Activated charcoal or activated carbon is carbon material that has been processed to contain many large pores creating a very large surface area.( )arrow_forward
- One method for the quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is area normalization. Here, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction involves dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2 and 30.2, in order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78 and 0.88, respectively.arrow_forwardThe sedimentation coefficient for a protein in solution is determined to be 4.3 S, and the diffusion coefficient to be 5.5x10-11 m2s-1 (vb = 0.74 mLg-1; density = 1.0 gmL-1; and viscosity = 1.0x10-3 Pas-1). Use this information to calculate the molar mass (kgmol-1) of the protein. Assume temperature of 298 K.arrow_forwardA molecular exclusion column has a diameter of 7.8 mm and a length of 30 cm. The solid portion of the particles occupies 20% of the volume, the pores occupy 40%, and the volume between particles occupies 40%. (a) At what volume would totally excluded molecules be expected to emerge? (b) At what volume would the smallest molecules be expected? (c) A mixture of polymers of various molecular masses is eluted between 23 and 27 mL. What does this imply about the retention mechanism for these solutes on the column?arrow_forward
- In a microemulsion, effectively all of the surfactant resides at the interface between the phases present. Describe how this is different to an aqueous surfactant solution at concentrations above and below the cmc.arrow_forwardSketch a graph of the van Deemter equation (plate height versus flow rate). What would the curve look like if the multiple path term were 0? If longitudinal diffusion were 0? If the finite equilibration time were 0?arrow_forwardIn have narrow peaks are said to chromatography, a) a large plate height (H) b) a small plate height (H) c) a small number of theoretical plates (N)arrow_forward
- 4.Discussed the Purposed of the Golden rules in the practice of Forensic Chrmistry.arrow_forwardIn stationary chromatography, the distribution (K) is the main criterion for the division of species in the sample between the two functions of the system, which factors depend onarrow_forwardThe following data were obtained for four compounds separated on a 20 m capillary coloumn. Compound t,(min) w(min) A 8.04 0.15 B 8.26 0.17 C 8.43 0.18 (a)Calculate the number of theoretical plates for each compound and the average number of theoretical plates for the column. (b) calculate the average height of theoretical plates. (c) explain why it is possible for each compound to have a different number of theoretical plates?arrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning