Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Question
Chapter 27, Problem 27.19QAP
Interpretation Introduction
Interpretation:
The definition for the retention index and its method of determination is to be stated.
Concept introduction:
The retention index is used to determine the solutes from the chromatograms. It is derived from the mixture of solutes chromatogram. The solute must have two
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
A student performed a chromatography
experiment to determine the Rf value of an
unknown amino acid. The distances traveled by
the amino acid and the solvent line were 3.0 cm
and 8.5 cm, respectively. What is the Rf value for
this amino acid?
0.3 cm
O 0.35
1.8
5.5
0.85 cm
0.65
O 2.8
29. What exactly is relative retention and retention time? How do you solve problems like this?
Describe how to obtain a Retention Factor. What does the retention factor tell you?
Chapter 27 Solutions
Principles of Instrumental Analysis
Ch. 27 - Prob. 27.1QAPCh. 27 - Prob. 27.2QAPCh. 27 - Prob. 27.3QAPCh. 27 - Prob. 27.4QAPCh. 27 - Prob. 27.5QAPCh. 27 - Prob. 27.6QAPCh. 27 - Prob. 27.7QAPCh. 27 - Prob. 27.8QAPCh. 27 - Prob. 27.9QAPCh. 27 - What are hyphenated GC methods? Briefly describe...
Ch. 27 - Prob. 27.11QAPCh. 27 - Prob. 27.12QAPCh. 27 - Prob. 27.13QAPCh. 27 - Prob. 27.14QAPCh. 27 - Prob. 27.15QAPCh. 27 - Prob. 27.16QAPCh. 27 - Prob. 27.17QAPCh. 27 - Prob. 27.18QAPCh. 27 - Prob. 27.19QAPCh. 27 - The same polar compound is gas chromatographed on...Ch. 27 - Prob. 27.21QAPCh. 27 - Prob. 27.22QAPCh. 27 - Prob. 27.23QAPCh. 27 - Prob. 27.24QAPCh. 27 - Prob. 27.25QAPCh. 27 - Prob. 27.26QAPCh. 27 - Prob. 27.27QAPCh. 27 - Prob. 27.28QAPCh. 27 - Why is GSC not used nearly as extensively as GLC?Ch. 27 - Prob. 27.30QAP
Knowledge Booster
Similar questions
- Table 2: Absorbance Values of Standards and Unknowns. Sample Blank Standard Solution 1 Standard Solution 2 Standard Solution 3 Standard Solution 4 Standard Solution 5 Water Sample 1 Water Sample 2 Phosphate Concentration (in ppm) Y17 0 ppm 0.02 ppm 0.04 ppm 0.08 ppm 0.16 ppm 0.32 ppm Freeman Lake 0.001 0.325 0.292 0.413 0.315 0.039 0.054 0.049 Absorbance at 2= 690 nm Calculations: 1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes on the graph and provide the curve with a title. Use a linear trendline to generate a best fit line to your data. Label the graph with the equation and the R" value. Insert your labeled graph in the space below. Xarrow_forwardBriefly explain how cation exchange chromatography can used in protein separation?arrow_forward▪ In a blood alcohol analysis by GC-FID, methanol elutes at 1 min, ethanol at 2 min, IPA at 3 min, and IS at 4 min. In the patient sample, the analyte elutes t 2 min and the IS at 8 min. What is the relative retention time and the analyte in the patient? A) methanol B) Ethanol chu C) IPAarrow_forward
- In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.arrow_forwardWhy chromatography is an important tool for analysis of organic compounds?arrow_forward1) a)What is affinity chromatography and what is it used for? b) A drug called Bamlanivimab is used to treat infections with SARS-CoV-2 (Covid-19).This drug was developed by isolating antibodies from the serum of a patient who recovered from Covid-19.What is possibly on the stationary phase in a column used to isolate antibodies to SARS-CoV-2? How are the antibodies eluted off the column to isolate them for drug development?arrow_forward
- How long is a macrocentury, if a lecture period (50.0 min) is close to 1.00 microcentury?arrow_forwardWhat is the best Chromatography instrument for analysis of Soluble Protein molecules CG-FID GC-MS LC-MS IC-MS O IC-ECarrow_forwardThe concentration of methylene blue in the sample is estimated through (select the correct) options: refractive index measurement (refractometry). turbidity measurement (turbidimetry). absorbance measurement (spectrophotometry). titration with 2% HCl solution.arrow_forward
- Use the calibration curve or linear regression equation to determine the concentration of FD&C blue dye No. 1 in Kool-aid. Food Coloring is 0.026 M in FD&C blue dye No. 1 7.5 x 10 -5 M is 29 mL -> 1000 mLarrow_forwardMacmillan Learning Complete the Ksp expression for Ag₂CO3. Ksp = 8.46 × 10-12 = MacBook Pro Search or type URLarrow_forwardPlease propose a chromatography-based strategy to purify human insulin proteins that have been expressed in bacterial cells.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning