Interpretation:
Percentages of each component should be calculated.
Concept introduction:
Area normalization method is used in quantitative analysis of compounds in chromatography. In this method of quantification, complete elution of all analytes in the sample is required. Following formulas are required for the calculation.
Answer to Problem 27.26QAP
Explanation of Solution
Given information:
| Compound | Relative peak area | Relative detector response |
| A | 32.5 | 0.70 |
| B | 20.7 | 0.72 |
| C | 60.1 | 0.75 |
| D | 30.2 | 0.73 |
| E | 18.3 | 0.78 |
Corrected areas of compound can be calculated as follows.
Now, the percentage of compound can be calculated as follows:
Or,
Also,
Thus,
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Chapter 27 Solutions
Principles of Instrumental Analysis
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- A standard sample contains 15.0 mM of analyte and 25.0 mM of internal standard. The analysis of the standard sample gives peak areas for the analyte and internal standard of 1.782 and 3.539, respectively, in a chromatography analysis. Then sufficient internal standard is added to a sample to make its concentration 10.0 mM. The analysis of this new sample yields peak areas for the analyte and internal standard of 0.894 and 2.981, respectively. Name the calibration method and report the analyte's concentration in the new sample. (e)arrow_forwardThe solid material in column chromatography is typically referred to as: O 1) the stationary phase 2) the mobile phasearrow_forwardWhich statement(s) are correct for gas chromatography? A) the components in a mixture can be identified from their retention time. B) the relative peak areas give the proportions of components in a mixture. C) Calibration curves are used to confirms the concentrations of components in a mixture.arrow_forward
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