Organic Chemistry
6th Edition
ISBN: 9781936221349
Author: Marc Loudon, Jim Parise
Publisher: W. H. Freeman
expand_more
expand_more
format_list_bulleted
Question
Chapter 27, Problem 27.15P
Interpretation Introduction
Interpretation:
The average yield of each of the
Concept introduction:
The term DNA stands for deoxyribonucleic acid. Deoxyribonucleic acid is the most important
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Which statement best explains the chemical differencesbetween DNA and RNA? (a) DNA has two different sugarsin its sugar–phosphate backbone, but RNA only has one.(b) Thymine is one of the DNA bases, whereas RNA’s correspondingbase is thymine minus a methyl group. (c) TheRNA sugar–phosphate backbone contains fewer oxygenatoms than DNA’s backbone. (d) DNA forms double helicesbut RNA cannot.
Q23:
Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.1% agarose gel, where the percentage is determined as mass/vol.
How many grams of agarose must she add to 413 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals)
**Assume the dissolution of agarose will not alter the final volume of the solution**
The following data were recorded from an experiment to measure the activity of a peptidase
enzyme. A number of solutions of different concentrations of a dipeptide were prepared then the
same small amount of the enzyme was added to each of them. The rate of cleavage of the peptide
bond was measured and the initial rate of the reaction was recorded
Peptide conc
(mmol L-¹)
0.334
0.450
0.667
1.00
1.33
1.67
Initial rate
0.152
0.201
0.269
0.417
0.505
0.667
(mmol L¹ s¹)
For the peptidase enzyme find the maximum velocity and the Michaelis constant.
Chapter 27 Solutions
Organic Chemistry
Ch. 27 - Prob. 27.1PCh. 27 - Prob. 27.2PCh. 27 - Prob. 27.3PCh. 27 - Prob. 27.4PCh. 27 - Prob. 27.5PCh. 27 - Prob. 27.6PCh. 27 - Prob. 27.8PCh. 27 - Prob. 27.9PCh. 27 - Prob. 27.10PCh. 27 - Prob. 27.11P
Ch. 27 - Prob. 27.12PCh. 27 - Prob. 27.13PCh. 27 - Prob. 27.14PCh. 27 - Prob. 27.15PCh. 27 - Prob. 27.16PCh. 27 - Prob. 27.17PCh. 27 - Prob. 27.18PCh. 27 - Prob. 27.19PCh. 27 - Prob. 27.20PCh. 27 - Prob. 27.21PCh. 27 - Prob. 27.22PCh. 27 - Prob. 27.23PCh. 27 - Prob. 27.24PCh. 27 - Prob. 27.25PCh. 27 - Prob. 27.26PCh. 27 - Prob. 27.27PCh. 27 - Prob. 27.28PCh. 27 - Prob. 27.29PCh. 27 - Prob. 27.30PCh. 27 - Prob. 27.31PCh. 27 - Prob. 27.32PCh. 27 - Prob. 27.33PCh. 27 - Prob. 27.34PCh. 27 - Prob. 27.35PCh. 27 - Prob. 27.36PCh. 27 - Prob. 27.37PCh. 27 - Prob. 27.38PCh. 27 - Prob. 27.39PCh. 27 - Prob. 27.40PCh. 27 - Prob. 27.41PCh. 27 - Prob. 27.42PCh. 27 - Prob. 27.43APCh. 27 - Prob. 27.44APCh. 27 - Prob. 27.45APCh. 27 - Prob. 27.46APCh. 27 - Prob. 27.47APCh. 27 - Prob. 27.48APCh. 27 - Prob. 27.49APCh. 27 - Prob. 27.50APCh. 27 - Prob. 27.51APCh. 27 - Prob. 27.52APCh. 27 - Prob. 27.53APCh. 27 - Prob. 27.54APCh. 27 - Prob. 27.55APCh. 27 - Prob. 27.56APCh. 27 - Prob. 27.57APCh. 27 - Prob. 27.58APCh. 27 - Prob. 27.59APCh. 27 - Prob. 27.60APCh. 27 - Prob. 27.61APCh. 27 - Prob. 27.62APCh. 27 - Prob. 27.63APCh. 27 - Prob. 27.64APCh. 27 - Prob. 27.65APCh. 27 - Prob. 27.66APCh. 27 - Prob. 27.67APCh. 27 - Prob. 27.68APCh. 27 - Prob. 27.69APCh. 27 - Prob. 27.70APCh. 27 - Prob. 27.71APCh. 27 - Prob. 27.72APCh. 27 - Prob. 27.73APCh. 27 - Prob. 27.74APCh. 27 - Prob. 27.75APCh. 27 - Prob. 27.76APCh. 27 - Prob. 27.77APCh. 27 - Prob. 27.78APCh. 27 - Prob. 27.79APCh. 27 - Prob. 27.80APCh. 27 - Prob. 27.81APCh. 27 - Prob. 27.82APCh. 27 - Prob. 27.83AP
Knowledge Booster
Similar questions
- 1. How does real-time polymerase chain reaction work? 2. What does the melting point represent?arrow_forward. Polyglycine, a simple polypeptide, can form a helix with 6 = -80°, ự = +150°. From the Ramachandran plot (see Figure 6.11), describe this helix with respect to (a) handedness and (b) number of residues per turn.arrow_forwardDetermine the oxidation states for each atom indicated in the structure of alanine below.arrow_forward
- Concentration Sof nicotinamide mono- nucleotide, in mM 0.138 0.220 0.291 0.560 0.766 1.460 a. 0.6482 b. 0.5853 0.4129 d. 0.4682 e. 0.9214 Initial rate vof nicotinamide- adenine dinucleotide formed, in micromoles Construct the Lineweaver-Burk linear plot and find the value of v C. 0.148 0.171 0.234 0.324 0.390 0.493 worksheet 16-Go... Microsoft Excel (P TSUarrow_forwardGive the sequence of the following tetrapeptide:arrow_forwardOn complete hydrolysis, a polypeptide gives two alanine, one leucine, one methionine, one phenylalanine, and one valine residue. Partial hydrolysis gives the following fragments: Ala-Phe, Leu-Met, Val-Ala, Phe-Leu. It is known that the first amino acid in the sequence is valine and the last one is methionine. What is the complete sequence of amino acids?arrow_forward
- 4) Paxlovid is treatment for COVID-19 that was recently approved for use because it can drastically reduce the risk for hospitalization and death when taken early in the infection. It is actually a package of two different antiviral drugs. One of those is Nirmatrelvir, which is a modified tripeptide protein mimetic designed to covalently bind to and inhibit the main protease (MPro) from the SARS-CoV-2 virus. This protease recognizes and hydrolyzes peptide bonds at specific points in their sequence, and it is essential for viral replication. a. Below is one of the sequences that Mpro recognizes and cleaves. It cleaves the peptide bond between the glutamine and the glycine. Draw out the structure of a peptide with this sequence, including the appropriate stereochemistry for biological amino acids and assuming the peptide is at physiological pH (pH = 7.4). SGVTFQGKF b. What would the net charge of the peptide be at physiological pH?. c. At what pH would the peptide be net neutral?arrow_forwardSketch the structures of ribose and deoxyribose ‐‐‐ what is the structural difference? Which nucleotides are associated with DNA? Which ones with RNA? Why must detergent be added to liberate the DNA? What parts of the cell contain DNA?arrow_forwardRetroviruses like HIV, the pathogen responsible for AIDS, incorporate an RNA template that is copied into DNA duringinfection. The reverse transcriptase enzyme that copies RNA into DNA is relatively nonselective and error-prone, leadingto a high mutation rate. Its lack of selectivity is exploited by the anti-HIV drug AZT (3′@azido@2′,3′@dideoxythymidine),which becomes phosphorylated and is incorporated by reverse transcriptase into DNA, where it acts as a chain terminator.Mammalian DNA polymerases are more selective, having a low affinity for AZT, so its toxicity is relatively low.(a) Draw the structures of AZT and natural deoxythymidine.(b) Draw the structure of AZT 5′@triphosphate, the derivative that inhibits reverse transcriptase.*2arrow_forward
- indicate the RIGHT alternative: (a) The Zwitterion form of an amino acid exists only at a point pH value. (b) In a peptide bond there is free rotation at the C-N bond. (c) In a polypeptide, the terminal carboxyl group may be present in its amide form. (d) At a pH greater than pI, an amino acid tends to move towards the cathode in an electrophoresis. (e) At any pH below pI, the predominant form of an amino acid is negatively charged.arrow_forwardConsidering the following nucleic acid in the image: Is the nucelic acid part of DNA or RNA? Is the nucleobase a purine or a pyrimidine? And is the oxygen heterocycle pyranose or furanose?arrow_forwardA 5-carbon sugar (ribose or deoxyribose) forms the central molecule in a nucleotide. By convention, the carbon atoms in the sugar are numbered from the original carbonyl position on the chain using a number plus the prime symbol (‘). For example, a nitrogenous base is attached to the 1’ (pronounced “one prime”) carbon position, which was originally the sugar’s carbonyl group. A phosphate group is attached to the 5’ carbon position, the carbon atom that is outside the sugar ring.Refer to the structure of a nucleotide and select the statement which is incorrect:arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Organic ChemistryChemistryISBN:9781305580350Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. FootePublisher:Cengage LearningChemistry: Matter and ChangeChemistryISBN:9780078746376Author:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl WistromPublisher:Glencoe/McGraw-Hill School Pub CoIntroductory Chemistry: An Active Learning Approa...ChemistryISBN:9781305079250Author:Mark S. Cracolice, Ed PetersPublisher:Cengage Learning
- Chemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningChemistry for Today: General, Organic, and Bioche...ChemistryISBN:9781305960060Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. HansenPublisher:Cengage Learning
Organic Chemistry
Chemistry
ISBN:9781305580350
Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote
Publisher:Cengage Learning
Chemistry: Matter and Change
Chemistry
ISBN:9780078746376
Author:Dinah Zike, Laurel Dingrando, Nicholas Hainen, Cheryl Wistrom
Publisher:Glencoe/McGraw-Hill School Pub Co
Introductory Chemistry: An Active Learning Approa...
Chemistry
ISBN:9781305079250
Author:Mark S. Cracolice, Ed Peters
Publisher:Cengage Learning
Chemistry: Principles and Reactions
Chemistry
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:Cengage Learning
Chemistry for Today: General, Organic, and Bioche...
Chemistry
ISBN:9781305960060
Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. Hansen
Publisher:Cengage Learning