Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 24.1, Problem 1COMQ
A DNA microarray is a slide that is dotted with
a. mRNAs from a sample of cells.
b. fluorescently labeled cDNA.
c. known sequences of DNA.
d. known cellular proteins.
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A contig is
a. a set of molecular markers used in gene mapping.
b. a set of overlapping fragments that form a continuous stretch of DNA.
c. a set of fragments generated by a restriction enzyme.
d. a small DNA fragment used in sequencing.
A DNA microarray is a slide that is dotted witha. mRNAs from a sample of cells.b. fluorescently labeled cDNA.c. known sequences of DNA.d. known cellular proteins.
In next-generation sequencing, which of these advances allows for massively parallel sequencing?
a. Pieces of DNA are fixed to a surface, so we can tell which new nucleotides were added to each piece.
b. DNA sequences are read in real-time as nucleotides are added to each piece.
c. Each segment of the genome can be pieced back together through shotgun alignment
d. Single molecules of DNA can be read without the need for amplification.
Chapter 24 Solutions
Genetics: Analysis and Principles
Ch. 24.1 - 1. A DNA microarray is a slide that is dotted...Ch. 24.1 - 2. The purpose of a ChIP-chip assay is to...Ch. 24.1 - 3. For the method of RNA sequencing (RNA-Seq),...Ch. 24.1 - A gene knockout is a gene a. whose function has...Ch. 24.2 - Prob. 1COMQCh. 24.2 - Prob. 2COMQCh. 24.2 - Prob. 3COMQCh. 24.2 - Prob. 4COMQCh. 24.3 - Prob. 1COMQCh. 24.3 - 2. Homologous genes
a. are derived from the same...
Ch. 24.3 - Prob. 3COMQCh. 24 - 1. Give the meanings of the following terms:...Ch. 24 - Prob. 2CONQCh. 24 - What is a database? What types of information are...Ch. 24 - Prob. 4CONQCh. 24 - Prob. 5CONQCh. 24 - Prob. 6CONQCh. 24 - Prob. 7CONQCh. 24 - Prob. 8CONQCh. 24 - Prob. 1EQCh. 24 - In the procedure called RNA sequencing (RNA-Seq),...Ch. 24 - 3. Can two-dimensional gel electrophoresis be used...Ch. 24 - Prob. 4EQCh. 24 - 5. Describe the two general types of protein...Ch. 24 - 6. Discuss the bioinformatics approaches that can...Ch. 24 - 7. What is a motif? Why is it useful for computer...Ch. 24 - Discuss why it is useful to search a database to...Ch. 24 - Prob. 9EQCh. 24 - In this chapter, we considered a computer program...Ch. 24 - Prob. 11EQCh. 24 - Prob. 12EQCh. 24 - Prob. 13EQCh. 24 - Refer to question 3 in More Genetic TIPS before...Ch. 24 - Prob. 15EQCh. 24 - Prob. 16EQCh. 24 - 1. Let’s suppose you are in charge of organizing...
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- Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardA DNA library is aa) A DNA fragment inserted into a vector.b) A general collection of all genes sequenced thus far.c) All DNA fragments identified with a probe.d) A collection of DNA fragments that make up the entire genome of a particular organism.arrow_forwardWhat is a cloning vector? A. The DNA probe used to locate a particular gene in the genome. B. An agent such as plasmid, used to transfer DNA from an in vitro solution into a living cell. C. The laboratory apparatus used to clone genes. D. An enzyme that cuts DNA into restriction fragments.arrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardPropose an experiment that can be done easily with a DNA microarray or RNA-seq that would have required years to do before these technologies were developed.arrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forward
- If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardRegarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genesarrow_forwardPut the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forward
- If you made a cDNA library from human brain cells and one from human liver cells would the clones in your two libraries be the same? a)Yes, the libraries would be identical. b)You cannot make cDNA libraries from these cell types. c) No, the libraries would have few overlapping clones. d) The libraries would have a number of similar clones for common cellular proteins but would have a number of unique clones associated specifically with each cell type.arrow_forwardSequencing a genome and identifying individual genes are processes typically carried out - A. via manual transcription by a team of genetic scientists. B. by computers using high-throughput methods. C. by hand with a team of information scientists. D. using experimental processes.arrow_forwardWhich type of genomics studies the transcripts and proteins expressed by a genome? a. Comparative genomics b. Structural genomics c. Proteogenomics d. Functional genomicsarrow_forward
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