Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 2.3, Problem 4RIA
Retrieve, Infer, Apply
4. What other differential staining procedures can be useful in identifying a bacterium? What information do they provide?
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Chapter 2 Solutions
Prescott's Microbiology
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1RIACh. 2.1 - Prob. 2RIACh. 2.1 - Prob. 3RIACh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1.1RIACh. 2.2 - Retrieve, Infer, Apply 2. If a specimen is viewed...Ch. 2.2 - Retrieve, Infer, Apply 3. How does resolution...
Ch. 2.2 - Prob. 1.4RIACh. 2.2 - Prob. 1.5RIACh. 2.2 - Retrieve, Infer, Apply 1. Briefly describe how...Ch. 2.2 - Prob. 2.2RIACh. 2.2 - Prob. 2.3RIACh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1RIACh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Retrieve, Infer, Apply 3. Explain what happens to...Ch. 2.3 - Retrieve, Infer, Apply 4. What other differential...Ch. 2.4 - Prob. 1MICh. 2.4 - Prob. 1RIACh. 2.4 - Describe in general terms how a TEM functions. Why...Ch. 2.4 - Prob. 3RIACh. 2.4 - Prob. 4RIACh. 2.5 - Prob. 1MICh. 2.5 - Retrieve, Infer, Apply Briefly describe each of...Ch. 2.5 - Prob. 2RIACh. 2 - Compare, Hypothesize, Invent 1. If you prepared a...Ch. 2 - Prob. 2CHICh. 2 - Compare, Hypothesize, Invent 3. How are freeze...Ch. 2 - Prob. 4CHI
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- Biochemical and differential tests can be incorporated into growth media or performed on isolated colonies after they have been cultured. Give three examples of a differential test that is incorporated into media and three examples that are applied after the colonies have been cultured.arrow_forwardAgarose Gel Electrophoresis Questions: 1. What determines how much agarose you should use in your gel? 2. Why do you see only DNA on the gel, and not protein? 3. How do you know which end of the gel to place the comb? 4. Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?arrow_forwardWhy is the looping out method preferable for sputum specimen for acid fast smears?arrow_forward
- Citrate test What critical enzyme allows the organism to acquire carbon from the medium?arrow_forwardtopic: gel electrophoresis What is the purpose of the running buffer?arrow_forwardVoges Proskauer test Does color of the medium change to red after both reagents were added and allowed to sit for 60 minutes? If yes, after about how many minutes before you can see red color?arrow_forward
- Microtome 1. Define microtome instrument 2. Mention the types of microtome instrument 3. What are the principle operation of any type of microtome? 4. Compare between two typo of microtome difference from principle operationarrow_forwardPlease answer fast Dilution Problem. A culture of Staphylococcus is diluted as follows: (1) 20mL are added to 80mL of water. (2) 10uL from (1) are then added to 9.99mL of water. (3) A 10-2 dilution is made from tube # (2). (4) 100uL from (3) are plated for a pour plate and incubated. Growth Problem.A culture with approximately 2x103 cells/mL were incubated. After 3 hours, the number of cells had increased to 3x105. a) How long was the generation time in minutes?b) How many generations have occurredarrow_forwardCause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstainingarrow_forward
- Explain the function of stacking gel, separating gel, and Coomassie blue stain in the electrophoresis experiment.arrow_forwardname 5 things about method staining.arrow_forwardExplain the rationale behind Macrohematocrit test (Wintrobe method) and Microhematocrit test (Clay adam's method).arrow_forward
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