Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 2, Problem 4CHI
Summary Introduction
Stimulated emission depletion in short STED, is the microscopy that provides super-resolution images. This kind of image is created by the selective deactivation of fluorophores. These molecules minimize the area of illumination at the focal point, through this method resolution of the given system is enhanced. This microscopy has been used for about 10 years.
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Students have asked these similar questions
List four specimens that are better viewed through a dissecting scope than a compound light microscope
Explain why parfocal imaging is important, especially when viewing very small specimens using the high power objective.
What description best describes the compound light microscope?
Uses a beam of electrons is transmitted through a specimen to form an image.
Has more than one lens and its own light source. In this type of microscope,
there are ocular lenses in the binocular eyepieces and objective lenses in a
rotating nosepiece closer to the specimen.
An optical imaging technique for increasing optical resolution and contrast of a
micrograph by means of using a spatial pinhole to block out-of-focus light in
image formation.
Designed for low magnification observation of a sample, typically
using light reflected from the surface of an object rather than transmitted
through it.
Part C
Once a slide is prepared and placed onto the microscope, the magnification and focus need to be altered. Describe how to change the magnification and bring the image into focus using a light microscope like the one in the simulation.
Chapter 2 Solutions
Prescott's Microbiology
Ch. 2.1 - Prob. 1MICh. 2.1 - Prob. 1RIACh. 2.1 - Prob. 2RIACh. 2.1 - Prob. 3RIACh. 2.2 - Prob. 1MICh. 2.2 - Prob. 2MICh. 2.2 - Prob. 3MICh. 2.2 - Prob. 1.1RIACh. 2.2 - Retrieve, Infer, Apply 2. If a specimen is viewed...Ch. 2.2 - Retrieve, Infer, Apply 3. How does resolution...
Ch. 2.2 - Prob. 1.4RIACh. 2.2 - Prob. 1.5RIACh. 2.2 - Retrieve, Infer, Apply 1. Briefly describe how...Ch. 2.2 - Prob. 2.2RIACh. 2.2 - Prob. 2.3RIACh. 2.3 - Prob. 1MICh. 2.3 - Prob. 1RIACh. 2.3 - Retrieve, Infer, Apply 2. Why would basic dyes be...Ch. 2.3 - Retrieve, Infer, Apply 3. Explain what happens to...Ch. 2.3 - Retrieve, Infer, Apply 4. What other differential...Ch. 2.4 - Prob. 1MICh. 2.4 - Prob. 1RIACh. 2.4 - Describe in general terms how a TEM functions. Why...Ch. 2.4 - Prob. 3RIACh. 2.4 - Prob. 4RIACh. 2.5 - Prob. 1MICh. 2.5 - Retrieve, Infer, Apply Briefly describe each of...Ch. 2.5 - Prob. 2RIACh. 2 - Compare, Hypothesize, Invent 1. If you prepared a...Ch. 2 - Prob. 2CHICh. 2 - Compare, Hypothesize, Invent 3. How are freeze...Ch. 2 - Prob. 4CHI
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- What is the importance of using an immersion oil in 100x magnification? Describe the changes in the field of view and the amount of available light when going from low to high power using the compound microscope. Give some limitations in the use of microscope. Conclusion about microscopearrow_forwardWhat is the purpose of using microscopes? If you need to move the microscope, describe how you should carry it. What three things change as you increase magnification? On the compound microscope, what power must be in place when you first find an image in themicroscope? On the compound microscope, which adjustment knob is used for bringing the specimen intofocus? On the compound microscope, which adjustment knob is used for sharpening the image of thespecimen after it is focused? On the compound microscope, which adjustment knob should you use on the high power? On the compound microscope, how do you change the objective lenses? What should be included when sketching or drawing what is seen through the microscope How do you properly use a microscope?arrow_forwardCareless Kris is using the microscope for the first time to look at cells and breaks a slide at high power. Explain to Kris the correct order of which objects lenses and focus knobs to use at each step when focusing a microscope from start to finish (hgih power).arrow_forward
- The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?arrow_forwardThe most widely used type of light microscope is thearrow_forwardCan someone answer and explain the following? What are the basic components of a Fluorescence Microscope and what are the functions of each? Are there any parts that you can remove without compromising accuracy and utility of the equipment? Can you suggest additional components to improve the equipment?arrow_forward
- The resolution of a microscope is affected by the wavelength of light used to examine the sample. O 1) True O 2) Falsearrow_forward3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?arrow_forwardDescribe 3 simple ways of preparing microscope slides for light microscopyarrow_forward
- List two ways that a stereoscopic dissecting microscope differs from a compound microscopearrow_forwardIn microscopy, why are different stains used for different samples? Be specific.arrow_forwardExplain the differences that you have seen using types of microscopes such as scanning electron, low power, high power, and oil immersion.arrow_forward
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