name 5 things about method staining

1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain?  2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?

Which of the following reasons is why slides should be labeled with a wax pencil before staining? (All three correct answers must be chosen to receive credit) Select All That Apply A To identify which bacterium/bacteria are placed on the slide B To prevent the slide from breaking during heat fixing ☐ C To identify which staining technique is being used OD To ensure the slide is in the correction orientation when placed on the stage ☐ E To give a focusing reference before attempting to find the bacterial smear ?

What is the advantage of gram staining over simple staining

Differentiate between the “hot method” and “cold method” of acid-fast staining.

What do you think there are 2 staining reagents applied in differential staining?

Write down the materials, reagents, Principle, procedure  and results for Ziehl-Neelsen stain

Could you describe what direct staining should look like when done to E. coli, M. luteus, and B. megaterium using microbiology termanology? Thanks!

explain the rationale or theoretical basis for differential staining procedures

Name the reagents used in Gram-staining and their roles in the staining procedure