Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstaining

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.4: The Gram Stain
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Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly:

1. Excessive heat was applied during fixation

2. Low concentration of crystal violet used during gram staining

3. Excessive washing between steps

4. Insufficient decolorization

5. Excessive counterstaining

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