Prescott's Microbiology
Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Chapter 2.2, Problem 2.3RIA
Summary Introduction

Fluorescent microscope is a kind of optical microscope, which uses phosphorescence as well as fluorescence to study the organic plus inorganic substances or samples. This microscopy excites a sample with a particular wavelength of light as well as forms an image that emits fluorescent light. Epifluorescent microscopy is the most commonly used kind of fluorescence microscopy.

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D) When performing fluorescence microscopy what are the stokes shift and why is it better  to have fluorochromes with a large stokes shift?  E) What is photobleaching and what is done when imaging histological samples to  overcome it when performing fluorescence microscopy?
Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?
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