The purpose behind the kanamycin, plant growth hormone and carbenicillin in the media, and the action shown in media when kanamycin is left out. Introduction: T- DNA (transfer deoxyribonucleic acid) of Agrobacterium tumefaciens is used for the production of transgenic plants. T- DNA is inserted in the chromosomal DNA of the plant as a vector for production of cloned genes. The vector gene is then transformed into the bacteria. The plant is then exposed to the bacteria. A rapid cell divisionfollows the infection of Agrobacterium . The cells of the plant are treated with kanamycin and carbenicillin. This agent is used in the media to kill Agrobacterium tumefaciens .

Question

Want to see the full answer?

Answer 1

Question

Chapter 22, Problem 4EQ

Summary Introduction

To review:

The purpose behind the kanamycin, plant growth hormone and carbenicillin in the media, and the action shown in media when kanamycin is left out.

Introduction:

T- DNA (transfer deoxyribonucleic acid) of Agrobacterium tumefaciens is used for the production of transgenic plants. T- DNA is inserted in the chromosomal DNA of the plant as a vector for production of cloned genes. The vector gene is then transformed into the bacteria. The plant is then exposed to the bacteria. A rapid cell divisionfollows the infection of Agrobacterium. The cells of the plant are treated with kanamycin and carbenicillin. This agent is used in the media to kill Agrobacterium tumefaciens.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Agrobacterium-mediated plant transformation is a method used to create transgenic plants. Agrobacterium tumefaciens, a soil bacterium works exceptionally well as a natural genetic engineer. Describe briefly the creation of a transgenic plant using A. tumefaciens with illustration.

Genetically modified foods are products produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Golden rice, Flavr Savr tomatoes and Roundup Ready soyabeans are products of this technology. Discuss the advantages of these three genetically modified crops. Name ONE (1) strategy to produce transformed plant cells.

You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a test group of strawberry plants. Select the events you would use to genetically engineer these plants. Insert the ice gene into the T-DNA region of a Ti plasmid; transform Agrobacterium tumefaciens with this plasmid; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. Insert the ice gene into the T-DNA region of the Agrobacterium tumefaciens chromosome; Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens; Select plant cells that have taken up the ice gene. O Insert the ice gene into a Ti transposon; Introduce the transposon into Agrobacterium tumefaciens Ti plasmid: Inoculate a culture of strawberry plant cells with Agrobacterium tumefaciens: Select plant cells that have taken up the ice gene. Insert the ice gene into a Ti bacteriophage; transduce Agrobacterium tumefaciens with this…