Human Biology (MindTap Course List)
11th Edition
ISBN: 9781305112100
Author: Cecie Starr, Beverly McMillan
Publisher: Cengage Learning
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Chapter 21, Problem 10SQ
Summary Introduction
Introduction: Recombinant DNA is synthesized as a part of
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Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only)
a. Clone
b. Plasmids
c. Biotechnology
a. PCR Amplification
b. Detection
c. Modified Trait
d. Human Genome
e. Genetic Modified Organism
Okazaki fragments are ________.
A.
short RNA primers needed for initiation of polymerization
B.
fragments of DNA polymerase I that lack 5' → 3' exonuclease activity
C.
short stretches of DNA formed on the lagging strand
D.
the smallest subunits of DNA polymerase III
The initial mechanism for repairing nucleotide errors in DNA is ________. a. mismatch repair b. DNA polymerase proofreading c. nucleotide excision repair d. thymine dimers
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- Chromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular vectors (such as plasmids) using __________. a. Ligase; Restriction Endonucleases. b. Restriction endonucleases; Ligase. c. DNA polymerase 1; Primase. d. DNA polymerase 1; Ligase. e. Primase; Helicase.arrow_forwardPart 1: Matching Match the term with its corresponding definition. a. Insertion e. Nonsense Mutation b. Deletion f. Missense Mutation c. Frameshift Mutation g. Germ-line Cells d. Silent Mutation h. Somatic Cells ____ 1. Remove a base or bases ____ 2. Add a base or bases ____ 3. Mutations in these cells are not passed to future generations but are passed to other body cells derived from them ____ 4. Mutation in which an amino acid is changed to a stop codon and the protein is truncated ____ 5. Mutations in these cells are passed to future generations ____ 6. Results if an insertion or deletion throws the reading frame of the gene message out of register ____ 7. Mutation where the protein does not change ____ 8. Mutation where a codon is changed resulting in another amino acid inserted in…arrow_forwarda. Pfu Polymerase b.dNTPs c.Buffer Match each component above to the correct function(s) listed below. Write your selection(s) for each component. You may have more than one answer for each. 1. unwinds DNA 2. synthesizes new DNA strands 3. enzymatically catalyzes Quikchange 4. nucleotide source for new DNA strands 5. Energy source for reaction(s) 6. Repairs errors in base pair matching 7. Maintains pH and salt levels 8. Creates polymer chainsarrow_forward
- . 1 · | 2. L 3 | 4 | . 5 6 7 Direction: Encircle the letter of the correct answer . 1. It is a double-stranded , self replicating, circular DNA molecule present in bacteria which is widely used as a gene cloning vector. A. Cosmid B. Plasmid. C. Phagemid. D. Genome 2. In plant genetic engineering, which of the following acts as vector? B. Gene of interest D. Ti-plasmid А. Agrobacterium lumefaciens. Receipt plant cell. Which of the following illustrates breeding? A farmer choose a breed of cow for greater milk production The use of bacteria in order to produce human insulin. The insertion of cloned genes to plant cells. All of the above. 3. А. В. С. D. Being pest resistance is one of the traits that are being introduced to plants like corn and eggplant with the insertion of Bt-toxin gene to plant cell. What method is use when a gene is inserted to plants? A. Biolistic C. Plasmid insertion by heat shock treatment 4. B. Electroporation D. All of these 5. A biotechnologist wants to enhance…arrow_forwardEnzymes used to cut genes in recombinant DNA research are known as ____. a. DNA polymerases b. transcriptases c. replicases d. ligases e. restriction enzymesarrow_forwardDefinition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified Organismarrow_forward
- n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genesarrow_forwardRestriction enzymes cut double-stranded DNA _____. a. at noncoding areas between genes b. between bacterial and viral DNAs c. at specific base sequences d. between purines and pyrimidines e. between promoter and operator DNA sequencesarrow_forwardMatch Column A with Column B F. v Analysis of genome-wide patterns of gene expression F. v Elucidation of patterns of coordinate gene regulation A. Recombinant DNA Technology B. Sanger sequencing |derives its power from the distinctive evolutionary patterns C. Restriction Enzymes and Ligase v involves joining a donor DNA fragment of interest to a vector D. GRNA of CRISPR technology Enzymes that cleave the DNA strands E. Restriction enzymes Enzymes used in Recombinant DNA Technology F. Functional Genomics homologous to target DNA that can base pair with it The first sequencing technology that uses amplification and fluorescent dyearrow_forward
- Bacterial ____ may be used as cloning vectors. a. polymerase b. endonuclease c. plasmids d. ligase e. transcriptasearrow_forwardMolecular hybridization A. Always occurs between 2 strands of DNA B. Happens at 94 degrees C C. Occurs during transformation D. Is mediated by DNA polymerase 1 E. Can be reproduced in situarrow_forwardDNA polymerase δ: DNA polymerase III as ________ a. Helicase: RPA b. DNA ligase: DNA polymerase I c. DNA polymerase alpha: primase d. DNA polymerase I: FEN-1/RNAse Harrow_forward
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